时间标签：数据日期 2026-11-01 · 生成时间 2026-11-01 05:30（Asia/Shanghai）｜ 检索窗口 PubMed edat 2026/10/31–2026/11/01 ｜ 情报发生日期 2026-11-01
研究问题
F2.0; 7 DEGs) and 2‐year male diapause (M0.5 vs.；M2.0; 8 DEGs), suggesting a conserved role in regulating inflammatory and immune‐modulatory processes during extended quiescence. 4
方法
To remove potential contamination from non‐arthropod sources (e.g., endosymbionts, soil microorganisms, or human‐derived sequences), we performed a decontamination step at the assembled transcript level.；Subsequent functional enrichment analyses—including GO term enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment—were performed on the DEG set.；Library construction was performed using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, USA), following the manufacturer's protocol.
核心结论
In females, farnesyl pyrophosphate synthase increased (TRINITY_DN896_c0_g1, log 2 FC = +1.436, FDR = 7.05 × 10 −15 ), consistent with continued investment in juvenile‐hormone precursor biosynthesis, whereas vitellogenin‐1‐like transcripts decreased after 1…；Transcriptomic and functional analyses reveal patterns consistent with systemic downregulation of energy metabolism pathways—particularly oxidative phosphorylation, glycolysis, and mitochondrial ATP synthesis—which collectively are associated with a state of…；Collectively, these results support a hierarchical and functionally integrated regulatory axis: environmental photoperiod is sensed and transduced via epigenetic (e.g., PBAP), neuroendocrine (e.g., JH), and circadian mechanisms, ultimately converging on the…
局限
Fifth, survival bias is an inherent limitation: individuals that survive extended diapause (e.g., 2 years) may represent a physiologically resilient subset, potentially biasing inferences about duration‐dependent molecular reprogramming.
对本项目的价值
人群纵向证据，可用于假说生成与终点选择：营养感知—自噬—蛋白稳态轴决定干预窗口与剂量方案，是组合疗法的时间锚。 ｜ 价值分 1.2/5（★☆☆☆☆）
全文来源
PubMed PMC JATS 全文（正文 54,082 字符，已过 DOI 逐字 + 题名指纹双重归属校验）
原文题录
- 英文标题：Molecular Adaptations and Sex-Biased Regulatory Pathways in Multi-Year Diapause of the Cabbage Beetle Colaphellus bowringi: A Duration-Resolved Transcriptomic Analysis.
- 期刊与标识：Ecology and evolution · PMID 42953526 · DOI 10.1002/ece3.74603
A 级证据分级：A 级（同行评议人体研究）
本文属人体研究证据，但需注意：观察性或小样本干预的结论不等于已证实临床获益；效应量与长期安全性仍需更大规模、更长随访的试验验证。
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