时间标签：数据日期 2026-10-31 · 生成时间 2026-11-01 05:30（Asia/Shanghai）｜ 检索窗口 PubMed edat 2026/10/28–2026/10/31 ｜ 情报发生日期 2026-10-31
研究问题
Diabetes Mellitus (DM), characterized by high blood sugar levels, is a serious chronic metabolic disease that is also frequently accompanied by hyperglycemia and various complications (including cardiovascular and chronic kidney diseases). , DM is becoming increasingly prevalent, according to the 2025 International Diabetes Federation Diabetes Atlas, approximately 589 million adults aged 20–79 years were living with diabetes worldwide in 2024, and this number is projected to increase to 853 million by 2050.；Among the two types of this metabolic disease defined as type 1 diabetes (T1DM) and type 2 diabetes (T2DM), T2DM is the most prevalent, and patients often suffer from wounds on the skin, most commonly on the lower extremities (especially the feet).
方法
The effects of the microneedle samples on modeled diabetic environment cell viability were evaluated using an indirect MTT assay.；For the viability assay, cells were seeded into 96-well plates at a density of 1 × 104 cells per well and incubated overnight to allow cell attachment.；The antioxidant and oxidant levels in the supernatants obtained from stressed HaCaT cells were determined using the Elabscience Total Antioxidant Status (TAS) Colorimetric Assay Kit (E-BC-K801-M) and the Elabscience Total Oxidant Status (TOS) Colorimetric Assay Kit (E-BC-K802-M), respectively, in accordance with the manufacturer’s instructions.；The migratory capacity of HaCaT human keratinocytes under simulated hyperglycemic conditions was assessed using a scratch wound gap closure assay.
核心结论
In contrast the characteristic lysine methylene resonance around 2.9 ppm, present in gelatin, was markedly reduced in GelMA, confirming that lysine residues were substituted by methacryloyl groups.；This high substitution degree falls within the range reported for GelMA depending on reaction conditions. , Higher GelMA substitution degrees correlate with enhanced mechanical strength, improved tip integrity during skin insertion, and greater structural stability under compression.；In the drug-loaded systems, characteristic peaks of the drugs were observed alongside the backbone spectra of GelMA or PLA. ‖ 结论：Collectively, these findings support the biological plausibility and multifunctional design rationale of the microneedle platform as a candidate strategy for diabetic wound management.
局限
However, these DSC results should be interpreted as supportive evidence rather than definitive proof of improved thermal stability.；These in vitro biocompatibility findings provide strong preliminary evidence supporting further in vivo evaluation of the developed system, particularly in terms of wound closure kinetics, histological tissue remodeling, and angiogenesis-related markers in diabetic wound models.；In summary, all groups exhibited sufficient penetration capacity in this preliminary parafilm model, supporting further evaluation in skin or skin-mimetic substrates, with the A-M-GelMA-MN group achieving the greatest penetration depth and consistency.
对本项目的价值
★☆☆☆☆ 代谢轴既干预敏感性最高，也是老药新用供给最充足的来源。
全文来源
PMC JATS 全文（正文 54,334 字符，已过标题/DOI 逐字归属校验）
原文题录
- 英文标题：3D-Printed GelMA Microneedles with Multilayer Therapeutic Properties for Diabetic Wound-Relevant Drug Delivery.
- 期刊与标识：ACS Omega · PMID 42959643 · DOI 10.1021/acsomega.6c06574
A 级证据分级：A 级（同行评议人体研究）
本文属人体研究证据，但需注意：观察性或小样本干预的结论不等于已证实临床获益；效应量与长期安全性仍需更大规模、更长随访的试验验证。
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