时间标签：数据日期 2026-10-31 · 生成时间 2026-11-01 05:30（Asia/Shanghai）｜ 检索窗口 PubMed edat 2026/10/28–2026/10/31 ｜ 情报发生日期 2026-10-31
研究问题
However, the therapeutic effect of MV on cigarette smoke-induced ALI has not been reported.
方法
CCK-8 assay was conducted using the CCK-8 kit (Dojindo, China).；Mice (C57BL/6J) (5–6 weeks old) were acquired from SPF (Beijing) Biotechnology Co., Ltd and randomly separated into five groups (5 mice/group).；This study was approved by the Animal Ethics Committee of the Aniphe Biolaboratory Inc.；Statistical analyses were conducted using GraphPad Prism 8, and the results were reported as the mean ± standard deviation from triplicates.
核心结论
We then cultured BEAS-2B cells and HUVECs separately on petri dishes, or co-cultured BEAS-2B cells and HUVECs using Transwell.；ELISA assay results showed that CSE exposure induced significantly higher levels of IL-18, IL-1β, and TNF-α in the co-culture group and the on-chip group than in the BEAS-2B group and HUVEC group, and there were no significant differences between the on-chip group and the co-culture group (Fig.；CSE significantly reduced the activity of bronchial epithelial BEAS-2B cells and human umbilical vein endothelial HUVEC cells, while MV increased the activity of the cells in a dose-dependent manner (Fig. ‖ 结论：These findings suggested that MV-induced autophagy may be dependent on the AMPK/mTOR signaling pathway.
局限
全文未设独立 limitations 节；结构上为非 RCT 设计，因果推断强度受限。
对本项目的价值
★☆☆☆☆ 营养感知与自噬轴决定「干预窗口 + 剂量方案」，是组合疗法的时间锚。
全文来源
PMC JATS 全文（正文 21,249 字符，已过标题/DOI 逐字归属校验）
原文题录
- 英文标题：Mogroside V regulates autophagy through AMPK/mTOR pathway to ameliorate cigarette smoke-induced acute lung injury.
- 期刊与标识：J Cardiothorac Surg · PMID 42958396 · DOI 10.1186/s13019-026-04556-1
A 级证据分级：A 级（同行评议人体研究）
本文属人体研究证据，但需注意：观察性或小样本干预的结论不等于已证实临床获益；效应量与长期安全性仍需更大规模、更长随访的试验验证。
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