数据日期 2026-10-29 · 生成时间 2026-10-29 06:03
证据级别：摘要级。本条依据 PubMed 结构化摘要与元数据撰写，付费墙全文未获取、未逐节核验，本站未做任何数值推算补全。
研究问题
Chronic circadian rhythm disruption, particularly constant light exposure (dark deprivation), is considered a factor that accelerates aging.
方法
However, the role of mitochondrial dysfunction in this process and the possibilities of its pharmacological correction remain insufficiently understood.
核心结论
The aim of this study was to perform a quantitative ultrastructural assessment of the mitochondrial apparatus of rat hepatocytes during aging under constant light exposure and with exogenous melatonin supplementation.；Male Wistar rats (n=480), starting from 3 months of age, were divided into three groups: control (10:14 h light/dark cycle), constant light group, and constant light group with melatonin added to drinking water (12 mg/L, 5 times/week).；At 6, 12, 18, and 24 months of age, electron microscopic examination of the liver was performed with assessment of numerical density, area, perimeter, cristae count, form factor (circularity, C), and mitochondrial inner membrane coefficient (MIMC).；Constant light exposure induced progressive accelerated mitochondrial aging: decreased numerical density, cristae number and length, organelle… ‖ 结论：Exogenous melatonin exerts a potent mitochondria-protective effect, stabilizing the ultrastructure and energy apparatus of hepatocytes, which substantiates its further investigation as a geroprotector in circadian disorders.
局限
未能获取全文，具体设计细节、失访与统计学处理无法核验。
对本项目的价值
★★★☆☆ 线粒体端点既是机制解释层，也是最容易拿到体外 / 影像读数的中间层。
原文题录
- 英文标题：[Quantitative electron microscopic assessment of the mitochondrial apparatus of hepatocytes during aging: Effect of constant illumination and melatonin correction.].
- 期刊与标识：Adv Gerontol · PMID 42935343 · DOI 10.34922/AE.2026.39.4.017**
B 级证据分级：B 级（同行评议临床前研究，主要为动物模型）
本文核心结论来自动物模型或体外细胞学，属临床前证据，不能外推至人体。「延长健康寿命」「改善功能」等表述在小鼠中的意义，与人类临床结局之间仍有距离。
关于本文的文献来源：本站依据 IMA 知识库「五刊监控·深度文献卡片」（SOP §4.2 摘要级）撰写，依据期刊公开摘要与 PubMed 元数据；付费墙全文未获取之处已标注「待补全文」，未做任何数值推算补全。