数据日期 2026-10-29 · 生成时间 2026-10-29 06:03
研究问题
Still, exactly how DHM connects antioxidant activity to mitochondrial quality control and metabolic remodeling in a living, aging organism remains poorly defined.
方法
Working solutions of 50, 100, and 200 μM DHM were prepared fresh in 0.5% (v/v) DMSO vehicle for every experimental condition.；The three concentrations tested fall within the range typically used to assess flavonoid bioactivity in this organism [21,22,23].；Lifespan Assay] Survival was measured in liquid culture following a protocol adapted from our prior work [23].；Synchronized L4-stage larvae (approximately 10 animals per well) were dispensed into 96-well plates containing S-complete medium, E. coli HB101 at the working concentration, 50 μM FUdR to block progeny production, and either DHM (50, 100, or 200 μM in 0.5% DMSO) or vehicle alone.
核心结论
DHM increased mean lifespan to 21.78 ± 0.32, 22.45 ± 0.33, and 22.96 ± 0.34 days at 50, 100, and 200 μM, respectively, corresponding to extensions of approximately 9.1%, 12.5%, and 15.0%.；Log-rank analysis confirmed significant survival benefits at all three concentrations (50 μM, 100 μM, and 200 μM, each p < 0.0001). ‖ 结论：Overall, redox-related, mitochondrial, and lipid-related readouts changed alongside the observed healthspan-related phenotypes; their causal relationships remain unresolved.
局限
It should also be noted that the cell-free DPPH/ABTS assays and the increased survival following acute juglone challenge demonstrate antioxidant capacity and stress resistance, respectively, but neither directly measures the in vivo redox or oxidative status of DHM-treated animals under basal aging conditions; direct in vivo markers of oxidative stress (e.g., protein carbonylation, lipid peroxidation, or ROS imaging) were not assessed in this study, and this is noted as a limitation of the present redox characterization.；As a partial, indirect check on mitochondrial content, the mtDNA copy number was also measured in a separate cohort and did not differ significantly from vehicle (p =…
对本项目的价值
★★★☆☆ 线粒体端点既是机制解释层，也是最容易拿到体外 / 影像读数的中间层。命中核心 线粒体；最大样本量约 418；设计 定性研究。
全文来源
PMC JATS 全文（正文 62,156 字符，已过标题/DOI 逐字归属校验）
原文题录
- 英文标题：Dihydromyricetin Is Associated with Healthspan-Related Phenotypes and Neuroprotection in Caenorhabditis elegans, Alongside Redox and Mitochondrial Quality-Control Changes.
- 期刊与标识：Antioxidants (Basel) · PMID 42939825 · DOI 10.3390/antiox15101251**
A 级证据分级：A 级（同行评议人体研究）
本文属人体研究证据，但需注意：观察性或小样本干预的结论不等于已证实临床获益；效应量与长期安全性仍需更大规模、更长随访的试验验证。
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