数据日期 2026-10-29 · 生成时间 2026-10-29 06:03
研究问题
Diabetic foot ulcers (DFU) remain a major clinical challenge due to their chronicity, high recurrence rates, and the risk of lower-limb amputation.
方法
After 24 h, the cells were pre-treated with the inhibitors for 30 min and then exposed to the compound VCE-005.1 for 6 h.；The inhibitors used included Dorsomorphin (DS) (AMPK inhibitor #S7306, Selleckchem, Houston, TX, USA), EX527 (SIRT1 inhibitor #E7034, Sigma-Aldrich, St.；Using a TriStar2 Berthold/LB942 multimode reader (Berthold Technologies, Bad Wildbad, Germany), luciferase activity was measured according to the instructions provided by the luciferase assay kit (Promega, Madison, WI, USA).；Western Blots] After stimulation and washing with phosphate-buffered saline (PBS), proteins were harvested using 50 μL of RIPA buffer containing phosphate and protease inhibitors (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 2 mM EDTA, 1% NP40, 0.5% sodium deoxycholate, 0.5 mM Na3VO4, 50 mM NaF, and 40 μg/μL Protease Inhibitor Cocktail) or with lysis buffer previously described by [18].
核心结论
A significant Treatment × Day interaction was observed in db/+ mice (p = 0.046), whereas no significant interaction was detected in db/db mice.；A significant treatment × day interaction was observed in db/+ mice (p = 0.040), whereas the interaction did not reach statistical significance in db/db mice (p = 0.061). ‖ 结论：The present study demonstrates that VCE-005.1 promotes diabetic wound healing through a coordinated mechanism involving endothelial protection, vascular regeneration and resolution of inflammation.
局限
Although several pro-angiogenic strategies, including growth factor- and cell-based therapies, have shown encouraging preclinical results, their clinical translation has been limited by modest efficacy, poor persistence at the wound site and safety concerns [1,2,3,4,5,40].；Although the precise molecular hierarchy remains to be established, our findings support a coordinated signaling network that promotes endothelial adaptation to metabolic and hypoxic stress.；The present study has several limitations that should be considered in the context of further development.
对本项目的价值
★★★☆☆ 血管老化提供可影像量化、可短期读取的中间终点，适合做机制到临床的桥接。命中核心 mTOR/AMPK；最大样本量约 6。
全文来源
PMC JATS 全文（正文 56,553 字符，已过标题/DOI 逐字归属校验）
原文题录
- 英文标题：Topical and Scaffold-Mediated Delivery of VCE-005.1 Enhances Diabetic Wound Healing Through Endothelial Protection and Vascular Regeneration.
- 期刊与标识：Pharmaceutics · PMID 42942733 · DOI 10.3390/pharmaceutics18101200**
B 级证据分级：B 级（同行评议临床前研究，主要为动物模型）
本文核心结论来自动物模型或体外细胞学，属临床前证据，不能外推至人体。「延长健康寿命」「改善功能」等表述在小鼠中的意义，与人类临床结局之间仍有距离。
关于本文的文献来源：本站依据 IMA 知识库「五刊监控·深度文献卡片」撰写，依据已获取的期刊全文（方法、样本量与结论已回到原文核对）；未能获取全文的同批条目另行标注「摘要级」，全程未做任何数值推算补全。