数据日期 2026-10-29 · 生成时间 2026-10-29 06:03
证据级别：摘要级。本条依据 PubMed 结构化摘要与元数据撰写，付费墙全文未获取、未逐节核验，本站未做任何数值推算补全。
研究问题
To characterize age-associated changes in lacrimal gland cellular composition, transcriptional states, and intercellular communication and to explore their relationship with lacrimal gland dysfunction and age-related dry eye disease (DED).
方法
Single-cell RNA sequencing (scRNA-seq) was performed on extraorbital lacrimal glands from young (12-week-old) and aged (20-month-old) male C57BL/6J mice.
核心结论
A total of 47,537 high-quality cells were analyzed using the Seurat pipeline.；Differential expression, pathway enrichment (gene set enrichment analysis), and cell-cell communication (CellChat) analyses were used to assess aging-associated changes in immune composition and signaling dynamics.；Aging was associated with pronounced immune-cell infiltration accompanied by epithelial and stromal remodeling, with higher observed representation of T cells, B cells, and plasma cells and broad transcriptional alterations across immune populations.；Regulatory T cells (Tregs) increased in frequency but exhibited transcriptional profiles suggestive of reduced suppressive capacity. ‖ 结论：These changes are associated with chronic inflammation and fibrosis and parallel mucosal immune aging observed in other mucosa-associated lymphoid tissues such as the gut and lung.
局限
未能获取全文，具体设计细节、失访与统计学处理无法核验。
对本项目的价值
★★☆☆☆ 方法学进展决定我们能否在有限样本下检出真实效应，属基础设施级收益。
原文题录
- 英文标题：Single-Cell Transcriptomic Analysis Reveals Age-Associated Cellular and Transcriptional Changes in Murine Lacrimal Glands.
- 期刊与标识：Invest Ophthalmol Vis Sci · PMID 42949068 · DOI 10.1167/iovs.67.12.73**
B 级证据分级：B 级（同行评议临床前研究，主要为动物模型）
本文核心结论来自动物模型或体外细胞学，属临床前证据，不能外推至人体。「延长健康寿命」「改善功能」等表述在小鼠中的意义，与人类临床结局之间仍有距离。
关于本文的文献来源：本站依据 IMA 知识库「五刊监控·深度文献卡片」（SOP §4.2 摘要级）撰写，依据期刊公开摘要与 PubMed 元数据；付费墙全文未获取之处已标注「待补全文」，未做任何数值推算补全。