时间标签：数据日期 2026-11-01 · 生成时间 2026-11-01 05:30（Asia/Shanghai）｜ 检索窗口 PubMed edat 2026/10/31–2026/11/01 ｜ 情报发生日期 2026-11-01
研究问题
Following surface modification, Mdivi-1 was incorporated into RVG-EVs using both passive incubation and incubation combined with mild sonication to enhance the loading efficiency.；Quantification of drug encapsulation indicated approximately 22.15 ± 6.28% with incubation alone, while incubation plus sonication significantly improved drug incorporation to 45.23 ± 8.81%, resulting in a higher encapsulation efficiency (…
方法
Cells were maintained in low-glucose DMEM containing 10% fetal bovine serum and 1% penicillin-streptomycin under standard incubation condition (37°C, 5% CO2).；Cells were seeded into 96-well plates at a density of 1×10 3 cells per well and maintained under standard culture condition for 24 h to allow attachment.；Oxygen-Glucose Deprivation Model of Ischemic Stroke To establish ischemic stroke environment in vitro, HT22 cells were exposed to OGD condition.
核心结论
Overall, these results confirm that RVG-EV-Mdivi-1 has good therapeutic effect for ischemic stroke, resulting in significantly improved neurological and behavioral outcomes compared to control groups.；Confocal laser scanning microscopy revealed that OGD/R exposure significantly reduced MMP, as indicated by diminished red JC-1 aggregate fluorescence and enhanced green monomer fluorescence ( ).；In vivo, we demonstrated that this strategy effectively enhanced brain delivery, attenuated mitochondrial dysfunction, and improved neurological recovery after ischemic stroke.；Normoxia shows stronger aggregates row and weaker monomers row; OGD-Control shows weaker aggregates row and stronger monomers row; treatment columns show increased aggregates row and reduced monomers row compared with OGD-Control.
局限
Some mechanistic and biodistribution analyses were performed using relatively small cohorts, warranting validation in larger sample size.；Future studies involving direct Mdivi-1 biodistribution, larger animal cohort and more detailed mechanistic validation are warranted.
对本项目的价值
干预级证据，可直接进入证据表并校准效应量参照：线粒体是衰老干预的共同下游效应器，可用于跨器官效应的统一读数。 ｜ 价值分 1.7/5（★★☆☆☆）
全文来源
PubMed PMC JATS 全文（正文 59,762 字符，已过 DOI 逐字 + 题名指纹双重归属校验）
原文题录
- 英文标题：Targeted Delivery of Mdivi-1 via RVG-Functionalized Extracellular Vesicles for Mitochondrial Protection in Ischemic Stroke.
- 期刊与标识：International journal of nanomedicine · PMID 42953445 · DOI 10.2147/ijn.s633788
A 级证据分级：A 级（同行评议人体研究）
本文属人体研究证据，但需注意：观察性或小样本干预的结论不等于已证实临床获益；效应量与长期安全性仍需更大规模、更长随访的试验验证。
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