时间标签：数据日期 2026-10-31 · 生成时间 2026-11-01 05:30（Asia/Shanghai）｜ 检索窗口 PubMed edat 2026/10/28–2026/10/31 ｜ 情报发生日期 2026-10-31
研究问题
DNA-based or genomic information is the only tag-less application that can trace product movements, given that an animal’s DNA remains constant and unique throughout its lifespan, even after slaughter, and at the point of sale or consumption.；However, most traceability systems to date have focused on SNP array genotyping or short-read sequencing (; ; ; ), which require laboratory processing and can take anywhere from two days to several weeks to obtain results.；The primary goal was to ascertain the feasibility of matching samples from the same animal acquired from distinct points in the supply chain, specifically: (1) Hair samples from the Farm, (2) Muscle samples from the abattoir, and (3) Muscle cut samples from unknown animals obtained from the Butcher Shop.
方法
Animal ethics approval was obtained from the University of Queensland ethics board (Animal Ethics Approval Number 2021/) The present investigation utilized a genomic sequencing approach to analyze a total of 46 samples obtained from 21 closely related Wagyu bovine specimens.；Each animal was subjected to two separate sequencing analyses, utilizing DNA from two distinct tissue types: Muscle and Hair.；Additionally, four muscle tissue samples (labelled Shop_02 to Shop_05), obtained from the original cohort of animals but with unknown individual identities, were sourced from the butcher shop and sequenced to serve as a validation group.；DNA concentrations were measured using the Qubit dsDNA Broad Range assay kit (Thermo Fisher Scientific).
核心结论
The read length N50 of muscle samples (12.97 ± 2.48 Kb) was significantly longer than that of hair samples (3.49 ± 0.83 Kb, P-value = 6.56 × 10−23) ().；Additionally, as presented in , the mapping rates of muscle samples (98.9 ± 0.2) were significantly higher than those of hair samples (94.2 ± 1.8, P-value = 3.81 × 10−13).；The number of called SNP sites increased with sequence coverage and plateaued above coverage of 8 (). ‖ 结论：While this study was conducted in a laboratory setting, the portability, flexibility, and rapid turnaround time of Oxford Nanopore sequencing suggest that an on-farm all-in-one assay could be feasible, subject to on-farm validation of the complete workflow together with cost and time analyses.
局限
The higher coverage from hair samples than from muscle samples suggests that further studies to expand the beef database for the traceability system could be conducted using hair samples, which are more easily obtained and transported and are routinely used by farmers and producers for genomic evaluation.；However, further studies to investigate our approach on-farm are warranted.；Using these unmapped reads, taxonomic classifications identified reads assigned to several microbial taxa, suggesting that the same sequencing dataset may provide preliminary information on non-host DNA signatures present in samples.
对本项目的价值
★☆☆☆☆ 菌群是可远程部署、成本最低的干预变量，但因果性与个体化响应仍是主要瓶颈。
全文来源
PMC JATS 全文（正文 26,028 字符，已过标题/DOI 逐字归属校验）
原文题录
- 英文标题：Simultaneous traceability and microbiome characterisation across beef supply chains with Oxford Nanopore sequencing.
- 期刊与标识：Transl Anim Sci · PMID 42948196 · DOI 10.1093/tas/txag160
A 级证据分级：A 级（同行评议人体研究）
本文属人体研究证据，但需注意：观察性或小样本干预的结论不等于已证实临床获益；效应量与长期安全性仍需更大规模、更长随访的试验验证。
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