数据日期 2026-10-29 · 生成时间 2026-10-29 06:03
研究问题
However, how senescence-associated changes in immune cells are linked to PAH progression remains unclear.
方法
Single-cell RNA-seq dataset GSE210248 was obtained from GEO, derived from dissected pulmonary artery/perivascular tissue, comprising 3 healthy donors and 3 PAH patients, with cell annotations provided by the original authors (18).；To assess the robustness of the senescence-associated score, an independent UCell rank-based scoring approach was applied using the same 321-gene set.；To assess the robustness of downstream analyses to the non-independence of repeated PHBI subjects, sensitivity analyses restricted to 189 unique subjects were also performed for GSEA, ssGSEA, and consensus clustering.；Differentially expressed genes (DEGs) between PAH patients and healthy controls were identified using the limma package (version 3.52.2) with thresholds of |log2FC| > 0.5 and adjusted P < 0.05 (32).
核心结论
(C) Volcano plot of DEGs between PAH and Controls (|log2FC| > 0.5, adjusted P < 0.05). ‖ 结论：Our findings suggest that a senescence-associated transcriptional signature in Mono.Macs may be linked to PAH and provide a prioritized candidate gene set for further investigation.
局限
The mechanisms underlying cellular senescence in PAH remain poorly elucidated, especially with respect to cell-type-specific senescence heterogeneity and its functional roles.；These findings highlight the importance of cell-type-specific validation in future studies.；These findings suggest potential transcriptomic heterogeneity among patients with PAH.
对本项目的价值
★★☆☆☆ 方法学进展决定我们能否在有限样本下检出真实效应，属基础设施级收益。最大样本量约 3,625；设计 单细胞、多组学。
全文来源
PMC JATS 全文（正文 78,007 字符，已过标题/DOI 逐字归属校验）
原文题录
- 英文标题：Integrative single-cell and bulk transcriptomic analysis identifies monocyte/macrophage senescence-associated candidate genes in pulmonary arterial hypertension.
- 期刊与标识：Front Immunol · PMID 42935721 · DOI 10.3389/fimmu.2026.1938985**
A 级证据分级：A 级（同行评议人体研究）
本文属人体研究证据，但需注意：观察性或小样本干预的结论不等于已证实临床获益；效应量与长期安全性仍需更大规模、更长随访的试验验证。
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