时间标签：数据日期 2026-10-31 · 生成时间 2026-11-01 05:30（Asia/Shanghai）｜ 检索窗口 PubMed edat 2026/10/28–2026/10/31 ｜ 情报发生日期 2026-10-31
研究问题
Despite these findings demonstrating upregulated energy metabolism in tumor‐educated platelets, the mechanisms by which platelet‐derived metabolic reprogramming influences HCC progression remain poorly understood.
方法
For Western blot analysis, the following antibodies were employed: mTOR (CST, USA, Cat#2983), phospho‐mTOR (Ser2448; CST, USA, Cat#5536), S6K1 (Zenbio, China, Cat#R380469), phospho‐S6K1 (Thr389/Thr412; Zenbio, China, Cat#310310), S6 (CST, USA, Cat#2217), phospho‐S6 (Ser235/236; CST, USA, Cat#2211), 4EBP1 (CST, USA, Cat#9644), phospho‐4EBP1 (Thr37/46; CST, USA, Cat#2855), and β‐actin (Zenbio, China, Cat#R380624).；Peripheral blood samples were obtained from healthy adult donors following informed consent under protocols approved by the Medical Ethics Committee of the First Affiliated Hospital of Guangxi Medical University (Ethical Approval No. 2025‐E0092).；After discarding the platelet‐depleted plasma, the platelet pellet was resuspended in phosphate‐buffered saline (PBS) containing 100 ng/mL PGE1, followed by a final centrifugation at 360 g for 20 min to obtain purified platelet suspensio
核心结论
Orthogonal partial least squares‐discriminant analysis (OPLS‐DA) revealed distinct metabolic clustering between platelet‐treated and untreated MHCC‐97H cells (Figure ), with 15 metabolites showing significant upregulation following platelet co‐culture (Figure ).；Pathway enrichment analysis of the top 25 metabolic pathways highlighted significant activation of the urea cycle and aspartate metabolism in platelet‐stimulated Huh7 cells (Figure ).；This analysis identified five significantly affected metabolic pathways, including the pentose phosphate pathway, nucleotide synthesis, TCA cycle, purine nucleotide cycle, and urea cycle. ‖ 结论：In summary, these results establish the leucine‐S6K1‐S6 signaling axis as a functional mediator of platelet‐stimulated HCC progression, highlighting dietary leucine modulation and S6K1 inhibition as potential therapeutic avenues to counteract platelet‐associated malignancy.
局限
However, our study has several limitations.；Furthermore, while downstream S6K1 inhibition in complete medium effectively recapitulated the phenotypes observed under leucine deprivation, future studies exploring dose‐dependent L‐leucine titration will provide further quantitative insights into the precise threshold required for platelet‐induced metabolic reprogramming.
对本项目的价值
★★☆☆☆ 干预证据决定参照标准效应量——任何抗衰干预的收益至少应对标该基线。最大样本量约 4,708,671。
全文来源
PMC JATS 全文（正文 34,453 字符，已过标题/DOI 逐字归属校验）
原文题录
- 英文标题：Dietary Leucine Restriction Suppresses Platelet-Driven HCC Growth by Attenuating mTOR Signaling.
- 期刊与标识：Cancer Sci · PMID 42952101 · DOI 10.1111/cas.70574
B 级证据分级：B 级（同行评议临床前研究，主要为动物模型）
本文核心结论来自动物模型或体外细胞学，属临床前证据，不能外推至人体。「延长健康寿命」「改善功能」等表述在小鼠中的意义，与人类临床结局之间仍有距离。
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