时间标签：数据日期 2026-10-31 · 生成时间 2026-11-01 05:30（Asia/Shanghai）｜ 检索窗口 PubMed edat 2026/10/28–2026/10/31 ｜ 情报发生日期 2026-10-31
研究问题
However, whether DOP confers protection against radiation-induced POI remains completely unexplored.；It remains unclear whether and how DOP orchestrates gut microbiota, PI3K/AKT signaling and follicular glycolysis-related processes to antagonize radiation-induced POI.；Therefore, this study aimed to investigate the protective effects of a partially purified DOP preparation against X-ray-induced POI in a mouse model and elucidate the underlying mechanisms involving the gut microbiota-PI3K/AKT-glycolysis axis.
方法
All animal procedures were approved by the Animal Ethics Committee of Youjiang Medical College for Nationalities (Approval No.: 2022010701) and performed in strict compliance with the NIH Guide for the Care and Use of Laboratory Animals.；Following 1 week of acclimatization, mice exhibiting regular estrous cycles were randomly assigned into three experimental groups (n = 10 per group) using a computer-generated randomization protocol: (1) Control group: received vehicle (saline) via oral gavage; (2) POI group (5 Gy): administered vehicle and subjected to a single dose of whole-body X-ray irradiation (5 Gy); (3) POI + DOP group (5 Gy + DOP): pretreated with the DOP preparation (500 mg/kg/day, dissolved in saline) via oral gavage for seven consecutive days prior to irradiation, with treatment continued for 14 days post-irradiation, and received the same irradiation protocol.
核心结论
Irradiation caused a transient decrease in food intake within the first 5 days, but no significant change in final body weight among groups ().；Although the transient reduction in food intake during the early post-irradiation period could have influenced metabolism or gut microbiota, the absence of a significant difference in final body weight suggests that nutritional status was largely restored.；However, irradiated mice (5 Gy group) exhibited severe estrous cycle disruption (prolonged diestrus or acyclicity), which was significantly improved by DOP pretreatment (). ‖ 结论：These findings indicate that DOP improves ovarian endocrine, histological, and molecular parameters associated with radiation-induced ovarian injury.
局限
Future studies incorporating tissue-level oxidative stress measurements would further strengthen this conclusion.；PKM2, in particular, is a rate-limiting enzyme in glycolysis and has been implicated in regulating cell proliferation and survival ().；While our data do not establish direct causality, we propose several potential mechanisms via the “gut-ovary axis” that are supported by our PICRUSt2-based functional prediction and correlation network analysis: (1) Reduced systemic inflammation: A healthy gut microbiota maintains intestinal barrier integrity, preventing translocation of lipopolysaccharide (LPS) and other bacterial products that trigger systemic low-grade inflammation and oxidativ
对本项目的价值
★☆☆☆☆ 菌群是可远程部署、成本最低的干预变量，但因果性与个体化响应仍是主要瓶颈。
全文来源
PMC JATS 全文（正文 39,659 字符，已过标题/DOI 逐字归属校验）
原文题录
- 英文标题：Dendrobium officinale polysaccharides ameliorate radiation-induced premature ovarian insufficiency through coordinated modulation involving the gut microbiota-PI3K/AKT-glycolysis axis.
- 期刊与标识：Front Pharmacol · PMID 42952587 · DOI 10.3389/fphar.2026.1891795
B 级证据分级：B 级（同行评议临床前研究，主要为动物模型）
本文核心结论来自动物模型或体外细胞学，属临床前证据，不能外推至人体。「延长健康寿命」「改善功能」等表述在小鼠中的意义，与人类临床结局之间仍有距离。
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