数据日期 2026-10-29 · 生成时间 2026-10-29 06:03
研究问题
Animal models play a pivotal role in elucidating the mechanisms underlying demyelination and impaired remyelination in MS.
方法
Authors complied with the ARRIVE guidelines for reporting data from animal experimentation [28].；[Animals] Female C57BL/6 J mice (n = 42) were obtained from Charles River (Sulzfeld, Germany).；Animals were allowed to adapt for 7 days prior to the start of an experiment.
核心结论
Female C57BL/6J mice aged 6 or 18 months at study onset were fed either normal food (NF) or cuprizone (0.2%) mixed into the diet for 5 weeks.；Notably, baseline T2-weighted signal intensity in the corpus callosum and other brain regions analyzed was significantly higher (p < 0.001) in 6-month-old compared with 18-month-old mice.；F-values for Time x Column Factor: Corpus callosum comprising the genu and the external capsule: F(13.39, 126.4) = 13.41, p < 0.0001; cortex: F (13.81, 130.4) = 1.458, p = 0.1369; olfactory bulb: F (14.41, 136.1) = 5.414, p < 0.0001; striatum: F(15.23, 143.9) = 3.718, p < 0.0001; thalamus: F (12.63, 119.3) = 0.8630, p = 0.5908; hippocampus: F (13.32, 125.8) = 0.8461, p = 0.6134; brainstem: F(13.48, 127.3) = 0.7298, p = 0.7361. ‖ 结论：Overall, the use of non-invasive MRI is well aligned with the 3Rs principles in animal experimentation [82].
局限
These findings therefore warrant further investigation into age-related increases in dystrophic neurites during normal brain aging.；In particular, the apparent dissociation between (i) the absence of T1-weighted signal changes despite pronounced demyelination of the cerebellar nuclei and (ii) the significant T1-weighted signal alterations observed in the olfactory bulb and cerebral cortex in the absence of corresponding changes in MTR or T2-weighted signal warrants further investigation.
对本项目的价值
★★★☆☆ 脑老化是最大未满足需求，也是终点最难量化的领域；biomarker 与认知读数选择最敏感。最大样本量约 42。
全文来源
PMC JATS 全文（正文 50,156 字符，已过标题/DOI 逐字归属校验）
原文题录
- 英文标题：Cerebellar nuclei remain vulnerable to cuprizone-induced demyelination in adult and aged mice: A longitudinal multimodal MRI-histology study.
- 期刊与标识：Aging Brain · PMID 42936164 · DOI 10.1016/j.nbas.2026.100171**
B 级证据分级：B 级（同行评议临床前研究，主要为动物模型）
本文核心结论来自动物模型或体外细胞学，属临床前证据，不能外推至人体。「延长健康寿命」「改善功能」等表述在小鼠中的意义，与人类临床结局之间仍有距离。
关于本文的文献来源：本站依据 IMA 知识库「五刊监控·深度文献卡片」撰写，依据已获取的期刊全文（方法、样本量与结论已回到原文核对）；未能获取全文的同批条目另行标注「摘要级」，全程未做任何数值推算补全。