时间标签：数据日期 2026-11-05 · 生成时间 2026-11-05 05:30（Asia/Shanghai）｜ 检索窗口 PubMed edat 2026/11/02 – 2026/11/06 ｜ 情报发生日期 2026-11-05
研究问题
In the present study, we investigated (i) the role of gut microbes in the metabolism of blueberry phytochemicals using germ-free mice and antibiotic-treated mice, (ii) the impact of depleting microbes on the vascular benefits of dietary blueberries, and (iii) the association between gut microbiome, blueberry metabolome, and vascular inflammation.2.Materials and methods2.1.Experimental designSix-week-old male C57BL/6J mice (Stock Number: 000664) were purchased from the Jackson Laboratory (Bar Harbor, ME, USA).；CB for relative abundance of taxa.3.5.Gut microbiota depletion reduces blueberry-derived metabolites and abolishes the benefits of dietary blueberries on vascular inflammationCohort 3 aims to determine whether the benefits of dietary blueberries on vascular inflammation are mediated through microbiome–metabolome interaction.；We assessed whether dietary blueberries affect metabolic parameters to determine whether the reduced vascular inflammation with blueberries are due to a secondary effect.
方法
In the present study, we investigated (i) the role of gut microbes in the metabolism of blueberry phytochemicals using germ-free mice and antibiotic-treated mice, (ii) the impact of depleting microbes on the vascular benefits of dietary blueberries, and (iii) the association between gut microbiome, blueberry metabolome, and vascular inflammation.2.Materials and methods2.1.Experimental designSix-week-old male C57BL/6J mice (Stock Number: 000664) were purchased from the Jackson Laboratory (Bar Harbor, ME, USA).；These mice were kept five per cage in a controlled environment with a 12-hour light/dark cycle, maintained at 23 ± 1 °C and 45 ± 5% humidity under artificial lighting.；Germ-free C57BL/6J mice were obtained from the Gnotobiotic Core Facility at the University of Utah.
核心结论
Additionally, 3,4-dimethoxycinnamic acid increased 60-fold in the CB group, but this increase was reduced by antibiotic treatment. p-Coumaric acid is present in many fruits and vegetables, but is not present in the blueberry powder used in our present study.27 Our findings suggest that p-coumaric acid may be produced by host enzymes, while hippuric acid and 3,4-dimethoxycinnamic acid could be generated by gut microbial enzymes.；Collectively, the genus enriched with blueberry supplementation is positively associated with blueberry-derived metabolites, whereas the genus reduced with blueberry supplementation is negatively associated with blueberry-derived metabolites.Fig. 2(A) Blueberry-derived plasma metabolites in the standard diet condition.；Metabolite analysis indicates that HFD decreases the levels of many circulating metabolites compared to mice fed a control diet (HF versus C mice) (Fig. 3B).
局限
However, Extibacter, which is reduced with blueberry supplementation, is negatively associated with hippuric acid and 3,4-dimethoxy cinnamic acid.；However, antibiotic treatment abolished the anti-inflammatory benefits conferred by dietary blueberries, and no significant differences in vascular endpoints were observed in HBA vs.；However, supplementation with blueberries and/or antibiotic treatment did not alter these metabolic parameters (Table S3).Fig. 3(A) Indices of vascular inflammation.
对本项目的价值
中高价值：属「微生物组及其代谢物干预」方向；微生物组及其代谢物干预栏目可用作本项目文献库与综述素材。 ｜ 价值分 3.0/5（★★★☆☆）
全文来源
PubMed PMC JATS（正文 39,395 字符，题名指纹 1.0，已过 PubMed PMCID 官方关联 + 题名指纹 + 正文长度归属校验）
原文题录
- 中文题名：蓝莓植物化学物经菌群-代谢物互作调节肠-血管轴
- 英文标题：Blueberry phytochemicals modulate the gut-vascular axis through microbiome-metabolome interaction.
- 期刊与标识：Food & function · PMID 42969403 · DOI 10.1039/d6fo04275a · PMC PMC13726404