时间标签：数据日期 2026-11-05 · 生成时间 2026-11-05 05:30（Asia/Shanghai）｜ 检索窗口 PubMed edat 2026/11/02 – 2026/11/06 ｜ 情报发生日期 2026-11-05
研究问题
To assess whether the relative distribution of T and B lymphocytes varied with age, the T/B lymphocyte ratio was compared among guinea pigs aged 2 months, 9 months, and 2–3 years.
方法
Recent developments based mainly on experimental research include several multi-color flow cytometry panels that allow detailed immunophenotyping of peripheral blood and tissue-associated immune cells, enabling the identification of major leukocyte populations such as monocytes, macrophages, B cells, and T cells in various tissues and blood samples in guinea pigs (13–15).In general, neoplasia is considered as one of the most common diseases in guinea pigs, particularly in animals older than 4 years.；These animals were assigned to a healthy control group (n = 13, age 2–3 years) or a presumptive lymphoma group (n = 9).；Blood samples were collected from the cranial vena cava as described by Jekl et al.
核心结论
The identity of the B-cell population was therefore confirmed by intracellular staining for the pan-B-cell marker CD79α (Table 1, panel 3), which enabled unequivocal identification of B lymphocytes, as shown in Supplementary Figure S4.Overall, the established flow cytometry panels enabled reliable discrimination of major leukocyte subsets across sample types and provided a consistent framework for downstream clinical analyses.；Detailed immunophenotypic analysis further demonstrated that T cells formed the predominant lymphocyte subset, representing 78.9% (44.6–89.1%) of lymphocytes, whereas B cells accounted for 6.3% (1.9–17.1%).；In cases in which affected tissue was also examined by flow cytometry, the tissue immunophenotype demonstrated marked B-cell predominance consistent with a B-cell lymphoid neoplasm.
局限
This is an important limitation, as guinea pigs are both widely used in research and are increasingly encountered in companion animal practice (1, 2).；Our results, however, differ from the age-associated hematological trends reported in previous studies.；Chronic antigenic stimulation or subclinical inflammatory disease may contribute to redistribution of lymphocyte subsets; however, these were ruled out based on history, clinical examination, negative parasitological results, and normal hematology and plasma chemistry analyses.
对本项目的价值
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全文来源
PubMed PMC JATS（正文 41,738 字符，题名指纹 0.93，已过 PubMed PMCID 官方关联 + 题名指纹 + 正文长度归属校验）
原文题录
- 中文题名：豚鼠白细胞免疫表型分析的实用流式细胞方法及其在淋巴系统肿瘤中的潜在应用
- 英文标题：A practical flow cytometric approach to leukocyte immunophenotyping in guinea pigs and its potential applications in lymphoid neoplasia.
- 期刊与标识：Frontiers in veterinary science · PMID 42974410 · DOI 10.3389/fvets.2026.1926791 · PMC PMC13726546