时间标签：数据日期 2026-10-31 · 生成时间 2026-11-01 05:30（Asia/Shanghai）｜ 检索窗口 PubMed edat 2026/10/28–2026/10/31 ｜ 情报发生日期 2026-10-31
研究问题
Our primary aim was to test whether whole-body VOC profiling identifies specific metabolic pathways perturbed during acute sepsis.
方法
All experimental protocols were approved by the institutional Animal Ethics Committee (Approval No.；LL20230715; July 13, 2023) and followed national guidelines for the care and use of laboratory animals.；Healthy male C57BL/6 mice (6–8 weeks old, 20–25 g) were housed under specific pathogen-free conditions (22 ± 2 °C, 50%–60% humidity, 12-h light/dark cycle) with free access to a standard rodent diet and water.；After 1 week of acclimatisation, mice were randomly allocated to a polymicrobial sepsis cohort induced by CLP (n = 14) or a sham-operated control cohort (n = 10).
核心结论
The permutation test used the 1,000 label permutations stated in the Methods; none of the 1,000 permuted datasets reached the observed R2Y or Q2 (permuted R2Y 0.491–0.895; permuted Q2 −1.410–0.508), giving P = 0.001 for both.；The submitted version reported P = 0.05, produced by the default permutation setting of the ropls function (20 permutations) rather than by the 1,000 permutations used here.；A significant permutation result does not make the model a validated classifier, however. ‖ 结论：While still pre-clinical, these findings support further development of volatile-based metabolic phenotyping in critical care.
局限
What is measured is therefore the sum of superimposed contributions: emissions from host metabolism, the gut microbiota, skin and fur, residual ambient air, and the chamber itself.；These results argue against the possibility that the pathway-level pattern is carried by halogenated contaminants, but they do not remove the underlying problem: quantitative blank subtraction was not performed, chamber blanks were used to classify compounds rather than to subtract them, and 16 of the 20 exogenous compounds were higher in the CLP cohort, so residual batch or chamber effects cannot be formally excluded.；Future work should randomise batches explicitly, subtract blanks quantitatively, and include a f
对本项目的价值
★☆☆☆☆ 代谢轴既干预敏感性最高，也是老药新用供给最充足的来源。最大样本量约 1,000。
全文来源
PMC JATS 全文（正文 27,249 字符，已过标题/DOI 逐字归属校验）
原文题录
- 英文标题：Whole-body volatile organic compound profiling reveals metabolic pathway perturbations in a murine polymicrobial sepsis model: a feasibility study.
- 期刊与标识：Front Med (Lausanne) · PMID 42953112 · DOI 10.3389/fmed.2026.1978491
A 级证据分级：A 级（同行评议人体研究）
本文属人体研究证据，但需注意：观察性或小样本干预的结论不等于已证实临床获益；效应量与长期安全性仍需更大规模、更长随访的试验验证。
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