数据日期 2026-10-29 · 生成时间 2026-10-29 06:03
研究问题
We previously demonstrated that a soy protein-based diet rich in phytoestrogens (i.e., “SOY”) reduces adiposity, adipose tissue inflammation, and IR in low aerobically fit female rats with and without ovarian function, yet whether the mechanism(s) for those benefits require E2 signaling remains unknown.
方法
Randomly cycling female (C57BL/6J background) αKO and βKO mice were pair-housed in a thermoneutral environment (26–28 °C) on a 12 h light/ 12 h dark cycle.；ERα wildtype (WT) controls were used to validate replication of previous studies [16,32] and underwent all treatments similar to the αKO and βKO mice.；All mice were initially provided with a low phytoestrogen content (i.e., “washout”) diet (150–250 mg/kg; #2018, Teklad Diets; Madison, WI, USA) and water ad libitum.；One week post-OVX mice were randomized to receive one of two custom-made diets: a phytoestrogen-free (CON; <15 mg/kg phytoestrogens) or a phytoestrogen-rich diet (SOY, 585 mg/kg phytoestrogens [genistein and daidzein; aglycone equivalents]) purchased from Harlan Laboratories Inc.
核心结论
SOY reduced final BW in the βKO mice by ~17%, which was not evident in the WT (0%) or αKO (6%) mice.；This associated with a trend toward lowered fat mass (↓39%; p = 0.07) in the βKO mice, whereas smaller reductions in WT (↓10%) and αKO (↓5%) did not approach statistical significance. ‖ 结论：Future studies should address mechanisms, including brain-mediated mechanisms that may help explain SOY’s protective effects on systemic metabolism and behavior, as well as perform these studies on aging male mice.
局限
Future studies should use a high-fat diet combined with SOY to determine whether SOY has anti-inflammatory effects in that setting.；However, the study was limited by the following: (1) an imperfect ERβ knockout model, which displays lack of ERβ DNA binding, but not non-genomic signaling pathways; (2) insufficient data to make definitive conclusions on WAT inflammatory populations; (3) lack of head-to-head comparison to young mice, and/or age-matched male mice, to truly assess and differentiate effects of sex and aging; and (4) lack of dose response curves and in vitro functional assays.
对本项目的价值
★★★★☆ 干预证据决定参照标准效应量——任何抗衰干预的收益至少应对标该基线。命中核心 线粒体；最大样本量约 12。
全文来源
PMC JATS 全文（正文 38,068 字符，已过标题/DOI 逐字归属校验）
原文题录
- 英文标题：Estrogen Receptor-Mediated Effects of a Phytoestrogen-Rich Soy-Based Diet on Metabolic Health Following Ovariectomy in Aged Mice.
- 期刊与标识：Nutrients · PMID 42943022 · DOI 10.3390/nu18203451**
A 级证据分级：A 级（同行评议人体研究）
本文属人体研究证据，但需注意：观察性或小样本干预的结论不等于已证实临床获益；效应量与长期安全性仍需更大规模、更长随访的试验验证。
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