数据日期 2026-10-29 · 生成时间 2026-10-29 06:03
研究问题
Moreover, the significant seroprevalence of LCMV within different populations across the world, including the United States, has raised the question of whether LCMV may contribute to the many cases of undiagnosed aseptic meningitis reported yearly [11,12].
方法
All cell lines, including Human A549 (ATCC CCL-185), were cultured in Dulbecco’s Modified Eagle Medium (DMEM; ThermoFisher Scientific, Waltham, MA, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS), 2 mM L-glutamine, 100 μg/mL streptomycin, and 100 U/mL penicillin (Cat.；A549 cells were seeded in 96-well clear-bottom plates (4.0 × 104 cells/well), treated with 2-fold serial dilutions of each compound, and incubated for 72 h.；Absorbance was measured using a Cytation 5 reader (BioTek, Agilent, Santa Clara, CA, USA), and values were normalized to vehicle (DMSO)-treated controls, set at 100%.；At 72 h post-infection, cells were fixed with 4% paraformaldehyde (PFA), and GFP or mCherry expression was measured by fluorescence using a Cytation 5 reader.
核心结论
Transfected cells were treated with cambinol (100 µM), vehicle control (VC), and VC containing 1% DMSO to match the DMSO concentration present in 100 µM cambinol generated from a 10 mM stock in 100% DMSO.；At 72 h post-transfection, cells were fixed with 4% PFA and stained with DAPI. ‖ 结论：Our findings contribute to a growing body of evidence supporting an important role of host sphingolipid metabolism in MaAv multiplication.
局限
Whether the pro-viral effect of Sirt-1 reflects its direct cytoplasmic functions during LCMV infection or its mediated effects on the expression of genes encoding host factors that support vRNP activity remains to be determined.；Whether the pro-viral role identified here for LCMV extends to other MaAvs remains to be determined.；Several limitations of the present work should be considered.
对本项目的价值
★★★☆☆ 相关范围内，作为背景与横向对照收录。命中核心 NAD⁺轴；最大样本量约 500,000；设计 单细胞。
全文来源
PMC JATS 全文（正文 41,554 字符，已过标题/DOI 逐字归属校验）
原文题录
- 英文标题：Sirtuin 1 Is Required for Optimal Mammarenavirus Multiplication.
- 期刊与标识：Viruses · PMID 42944008 · DOI 10.3390/v18101088**
A 级证据分级：A 级（同行评议人体研究）
本文属人体研究证据，但需注意：观察性或小样本干预的结论不等于已证实临床获益；效应量与长期安全性仍需更大规模、更长随访的试验验证。
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