时间标签：数据日期 2026-10-31 · 生成时间 2026-11-01 05:30（Asia/Shanghai）｜ 检索窗口 PubMed edat 2026/10/28–2026/10/31 ｜ 情报发生日期 2026-10-31
研究问题
Acute kidney injury (AKI) affects 13.3 million individuals annually worldwide, with a mortality rate exceeding 50% in critically ill patients.；Despite advances in supportive care, no targeted pharmacotherapies are available.
方法
Successful transfection was validated using western blot analysis.；Lactate dehydrogenase (LDH) release, a marker of cell membrane damage, was quantified using an LDH Cytotoxicity Assay Kit (Beyotime, Shanghai, China).；The LDH assay signal was assessed by recording the absorbance at 490 nm.；Animal procedures were approved by the Institutional Animal Care and Use Committee (IACUC) of Chongqing Medical University (approval no.
核心结论
Compared with the sham-operated control group, the proportion of cuproptosis increased significantly after ischemic injury ().；GO analysis revealed significant enrichment of cuproptosis-related biological processes after I/R injury, particularly those involving oxidative stress responses, cell-cell signaling by Wnt, regulation of Wnt signaling, stress-activated MAPK cascade, JAK-STAT ().；KEGG pathway analysis highlighted metal ion homeostasis and MAPK signaling as the most significantly altered pathways (), implicating copper-redox dysregulation in AKI pathogenesis. ‖ 结论：These findings provide a preclinical rationale for the repurposing of this natural product for the treatment of ischemic kidney disease.
局限
Third, these findings provide a preclinical rationale for strategies aimed at preserving physiological copper function and limiting pathological copper accumulation.；A limitation of our single-cell gene-set scoring approach is its inability to distinguish baseline metabolic gene expression from active cell death program activation.；Future studies employing orthogonal approaches—such as assessing DLAT oligomerization, iron-sulfur cluster protein stability, and FDX1-dependent functional rescue assays—are needed to differentiate cuproptosis execution from a state of copper susceptibility.
对本项目的价值
★☆☆☆☆ 代谢轴既干预敏感性最高，也是老药新用供给最充足的来源。设计 single-cell。
全文来源
PMC JATS 全文（正文 39,630 字符，已过标题/DOI 逐字归属校验）
原文题录
- 英文标题：Schisandrin B attenuates ischemic acute kidney injury in a CTR1-dependent manner by inhibiting cuproptosis.
- 期刊与标识：Front Cell Dev Biol · PMID 42959219 · DOI 10.3389/fcell.2026.1993380
A 级证据分级：A 级（同行评议人体研究）
本文属人体研究证据，但需注意：观察性或小样本干预的结论不等于已证实临床获益；效应量与长期安全性仍需更大规模、更长随访的试验验证。
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