数据日期 2026-10-29 · 生成时间 2026-10-29 06:03
研究问题
Tuberculosis is a chronic, consumptive infectious disease affecting both humans and animals caused by Mycobacterium tuberculosis (Mtb) [1].
方法
Drug Treatment] Resveratrol (Merck, Darmstadt, Germany) was dissolved in DMSO to prepare a 20 mM stock solution, aliquoted into 20 µL/tube, and diluted to 10 μM (low), 20 μM (medium), and 40 μM (high) concentrations before use.；EX527 (Purchased from AmBeed, Arlington Heights, IL, USA) was dissolved in DMSO to prepare a 10 mM stock solution, aliquoted into 10 µL/tube, and diluted to 10 μM concentrations before use.；Equal amounts of protein samples were separated by SDS-PAGE and subsequently transferred to a PVDF membrane using the wet transfer method.；The primary antibodies used included: LC3B (A19665), Beclin1 (A21191), Atg5 (A19677), Atg7 (A19604), p62 (A19700), Bcl-2 (A19693), Bax (A19684), Caspase 3 (A19654), Caspase 9 (A18676), PARP1 (A19612) (all from ABclonal, Wuhan, China), Ac-FOXO1 (Thermo, PA5-116749), FOXO1 (Biodragon, RM5634) and β-actin (Abways, Shanghai, China, AB0035).
核心结论
Compared with the BCG group, treatment with different doses of RES (L-RES, M-RES, H-RES) combined with BCG further upregulated the expression of LC3B-II and Beclin1 (L-RES group p < 0.05, M-RES group and H-RES groups p < 0.001) and inhibited p62 expression (L-RES group and M-RES group p < 0.05, H-RES groups p < 0.001). ‖ 结论：This study confirmed that BCG induces autophagosome accumulation and apoptosis in RAW264.7 cells independently of SIRT1.
局限
Future studies using primary macrophages and animal infection models are warranted to validate these findings and to determine their physiological relevance.；Future studies using SIRT1 overexpression rescue or CRISPR/Cas9-mediated SIRT1 knockout will be important to further confirm the specificity of these findings.；Future studies using tandem fluorescent LC3 reporters are needed to distinguish between these two possibilities.
对本项目的价值
★★★☆☆ 炎症衰老是几乎所有慢性老年病的公共通路，也是最有希望做"抗炎但保留免疫"解耦的靶点。最大样本量约 3。
全文来源
PMC JATS 全文（正文 43,644 字符，已过标题/DOI 逐字归属校验）
原文题录
- 英文标题：Research on the Regulatory Effects of Resveratrol on BCG-Induced Autophagy and Apoptosis in Macrophages via Targeting SIRT1.
- 期刊与标识：Microorganisms · PMID 42943603 · DOI 10.3390/microorganisms14102365**
B 级证据分级：B 级（同行评议临床前研究，主要为动物模型）
本文核心结论来自动物模型或体外细胞学，属临床前证据，不能外推至人体。「延长健康寿命」「改善功能」等表述在小鼠中的意义，与人类临床结局之间仍有距离。
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