时间标签：数据日期 2026-10-31 · 生成时间 2026-11-01 05:30（Asia/Shanghai）｜ 检索窗口 PubMed edat 2026/10/28–2026/10/31 ｜ 情报发生日期 2026-10-31
研究问题
In contrast, the diversity and functional potential of squid-associated microbial communities remain largely unexplored, despite increasing evidence that host-associated microbiota play fundamental roles in animal health, development, nutrition, and fitness.；However, it represents only a small fraction of the microbial diversity associated with cephalopods.；The taxonomic composition and organization of microbial communities inhabiting other squid tissues (including the skin, gills, gastrointestinal tract, and nidamental gland) remain poorly characterized.
方法
Negative controls were obtained by performing blank extraction (n = 2).；The V3-V4 region of the 16S rDNA gene was amplified using universal bacterial primers modified for Illumina sequencing: 341F*—CCTACGGGNGGCW GCAG/805R*—GACTACHVGGGTATC TAATCC (Klindworth et al. ).；The quality of forward and reverse reads was plotted and analyzed, then the sequencing adapters were removed from the reads.；Sequencing depth across samples ranged from 37 362 to 566 200 reads (median = 163 555 reads; IQR= 96 824–258 594 reads).
核心结论
Although the inclusion of two species provides broader ecological context, the dataset does not support valid interspecific comparisons or attribution of observed differences to host identity per se.；Finally, as our study relies on amplicon sequencing, functional roles attributed to taxa are based on literature inference rather than direct evidence; shotgun metagenomics or targeted genomics would help clarify the underlying genes and mechanisms.；In general, microbiome alpha diversity (as measured by the bacterial richness and the Shannon index) in the different organs of both species was equal to or lower than in the surrounding water (see Fig. ). ‖ 结论：Overall, our findings underscore the importance of organ-specific microbiomes in cephalopods and provide a foundational framework for understanding host-microbe interactions in these marine invertebrates.
局限
Because the two squid species were sampled at different locations, seasons and years, and because not all organs were collected from both species, host identity and environmental factors are fully confounded in this dataset.；This limitation is further compounded by the absence of environmental metadata, such as water temperature, salinity, dissolved oxygen and depth, which can strongly influence marine microbial communities.；Both Hyphomicrobiales and Ruegeria therefore warrant particular attention in future investigations.
对本项目的价值
★☆☆☆☆ 菌群是可远程部署、成本最低的干预变量，但因果性与个体化响应仍是主要瓶颈。
全文来源
PMC JATS 全文（正文 23,413 字符，已过标题/DOI 逐字归属校验）
原文题录
- 英文标题：Organ-specific microbiomes reveal strong bacterial compartmentalization in two tropical squid species.
- 期刊与标识：Microlife · PMID 42953723 · DOI 10.1093/femsml/uqag037
B 级证据分级：B 级（同行评议临床前研究，主要为动物模型）
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