时间标签：数据日期 2026-10-31 · 生成时间 2026-11-01 05:30（Asia/Shanghai）｜ 检索窗口 PubMed edat 2026/10/28–2026/10/31 ｜ 情报发生日期 2026-10-31
研究问题
However, oral delivery exposes phytochemicals to harsh gastrointestinal conditions and first-pass hepatic metabolism, which not only reduces the fraction reaching systemic circulation but also generates variable and unpredictable metabolite profiles [].；However, most existing systems rely on injection or wound dressing formats, underscoring the need for a minimally invasive, targeted delivery approach suitable for long-term management of chronic conditions like obesity.；As a result, there is a significant gap in the development of multi-target synergistic therapeutic approaches [].
方法
BCA Protein Assay Kit (P0011), dNTPs (D7373), Hoechst 33,342 (C1027), and sulfobutylether-β-cyclodextrin (SBE-β-CD, Y027108) were purchased from Beyotime Biotechnology.；Phi29 DNA polymerase (0.5 U/µL, D7053L, Beyotime) was then added, and isothermal amplification was performed at 30 °C for 24 h, followed by enzyme inactivation at 70 °C for 20 min.；Superoxide dismutase (SOD)-like activity was quantified using a Total SOD Activity Assay Kit (S0101S, Beyotime). 3T3-L1 and RAW264.7 cells were obtained from the Chinese Academy of Sciences Cell Bank.；Intracellular NAD⁺ and NADH levels were measured using an NAD⁺/NADH Assay Kit (S0175, Beyotime).
核心结论
In the presence of Mn²⁺, the abundant pyrophosphate (PPi⁴⁻) and phosphate (PO₄²⁻) groups generated during RCA reacted with Mn²⁺ to form manganese pyrophosphate/phosphate, which co-biomineralized with nucleic acids into flower-like MNF (Fig. a).；Compared with Mn-free NF, M2NF exhibited increased particle size and a decreased zeta potential, due to the enhanced RCA efficiency resulting from Mn²⁺ acting as a cofactor for Phi29 DNA polymerase (Figure S1).；When the Mn²⁺ concentration was increased to 4 mM, M4NF adopted a petal-like morphology with higher specific surface area. ‖ 结论：Collectively, this work presents a promising strategy for long-term obesity management that aims to promote favorable metabolic changes through a multi-pronged approach, simultaneously addressing impaired beiging, oxidative stress, and chronic inflammation, all within a single, patient-friendly platform.
局限
Given that MNF scavenges ROS and alleviates oxidative stress, we propose that reduced oxidative damage suppresses PARP1 expression, thereby limiting NAD⁺ consumption.；While our study demonstrates the therapeutic efficacy of the MNF-allicin microneedle system, several limitations should be acknowledged.；Future studies will directly measure glucose tolerance, insulin sensitivity and energy expenditure to fully characterize the systemic metabolic improvements conferred by this microneedle system.
对本项目的价值
★☆☆☆☆ 代谢轴既干预敏感性最高，也是老药新用供给最充足的来源。
全文来源
PMC JATS 全文（正文 48,698 字符，已过标题/DOI 逐字归属校验）
原文题录
- 英文标题：Synergistic promotion of white-to-beige adipocyte transition by manganese-nucleic acid framework and allicin for alleviating obesity.
- 期刊与标识：J Nanobiotechnology · PMID 42958258 · DOI 10.1186/s12951-026-04857-6
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