时间标签：数据日期 2026-10-31 · 生成时间 2026-11-01 05:30（Asia/Shanghai）｜ 检索窗口 PubMed edat 2026/10/28–2026/10/31 ｜ 情报发生日期 2026-10-31
研究问题
However, standard fasting protocols in frequently rely on experimental manipulations that independently influence lifespan and complicate the interpretation of dietary effects.；We tested three intermittent fasting protocols: 5 h ( ) and 6 h ( ) daily fasting initiated on day 1 of adulthood, and 18 h fasting ( ) initiated on day 3 of adulthood, after animals had reached their reproductive peak.；However, Li et al. acknowledge that the inclusion of FUdR limits their conclusions to relative lifespan comparisons performed under identical conditions.
方法
To compensate for elevated censoring associated with intermittent fasting, cohort sizes were set at 300 worms for fasting conditions and 200 worms for ad libitum controls.
核心结论
To prevent worms from seeding food-free agar with gut-retained or cuticle-bound E. coli , studies commonly employ bactericidal antibiotics (e.g., streptomycin or carbenicillin), UV- or heat-killed bacteria, or peptone-depleted media (Table 1) .；Consequently, the lifespan extension observed under these conditions may reflect responses to pharmacological or nutritional perturbations rather than physiological effects of fasting-refeeding cycles per se.；To minimize experimental confounders and build upon our previous studies of short-term fasting (Silva-García et al., 2013; Láscarez-Lagunas et al., 2014; Huelgas-Morales et al., 2016), we developed an adult-onset intermittent fasting regimen that requires no FUdR, antibiotics, or other bacterial killing agents, on either fasting or fed plates. ‖ 结论：Survival curves were compared using the log-rank (Mantel-Cox) method in GraphPad Prism.
局限
To minimize experimental confounders and build upon our previous studies of short-term fasting (Silva-García et al., 2013; Láscarez-Lagunas et al., 2014; Huelgas-Morales et al., 2016), we developed an adult-onset intermittent fasting regimen that requires no FUdR, antibiotics, or other bacterial killing agents, on either fasting or fed plates.；Prior to each transfer, animals were individually picked onto an adjacent unseeded agar surface or a separate unseeded NGM plate and allowed to crawl briefly to shed residual bacteria from the cuticle.
对本项目的价值
★☆☆☆☆ 干预证据决定参照标准效应量——任何抗衰干预的收益至少应对标该基线。
全文来源
PMC JATS 全文（正文 7,822 字符，已过标题/DOI 逐字归属校验）
原文题录
- 英文标题：A simplified intermittent fasting regimen robustly extends C. elegans lifespan without FUdR or antibiotic confounds.
- 期刊与标识：MicroPubl Biol · PMID 42953508 · DOI 10.17912/micropub.biology.002408
A 级证据分级：A 级（同行评议人体研究）
本文属人体研究证据，但需注意：观察性或小样本干预的结论不等于已证实临床获益；效应量与长期安全性仍需更大规模、更长随访的试验验证。
关于本文的文献来源：本站依据 IMA 知识库「五刊监控·深度文献卡片」撰写，依据已获取的期刊全文（方法、样本量与结论已回到原文核对）；未能获取全文的同批条目另行标注「摘要级」，全程未做任何数值推算补全。