时间标签：数据日期 2026-11-01 · 生成时间 2026-11-01 05:30（Asia/Shanghai）｜ 检索窗口 PubMed edat 2026/10/31–2026/11/01 ｜ 情报发生日期 2026-11-01
研究问题
However, the application of Huaier in the context of DN remains largely unexplored.；The present study systematically investigated the pharmacological effects and underlying mechanisms of Huaier in DN through in vitro and in vivo experiments, providing a theoretical basis for its potential clinical application. 2
方法
After the experiment, all animal carcasses were collected for harmless disposal. 2.3.1 Biochemical Analysis of Blood and Urine Samples Blood samples were centrifuged to separate the serum.；Visualization was achieved via chemiluminescence, and relative protein expression was analyzed using ImageJ software. 2.6 Statistical Analysis Data are expressed as mean ± SD from independent triplicates ( n ≥ 3).；Samples were subsequently incubated with primary antibodies targeting β‐catenin and Wnt5a (1:200; ABclonal) overnight at 4°C, followed by Alexa Fluor 488‐conjugated secondary antibodies (1:500; 1 h) and DAPI nuclear counterstaining.
核心结论
Compared with control cells, treatment of HK‐2 cells with HG/PA markedly increased cleaved caspase 3 expression but decreased the Bcl‐2/Bax ratio (Figure ).；The apoptotic rate of HK‐2 cells significantly increased following HG/PA stimulation, an effect that was reversed by Huaier treatment (Figure ).；TUNEL staining demonstrated significantly reduced apoptotic cell density in Huaier‐treated mice relative to untreated KKAy controls (Figure ).；Compared with that of the control group, Huaier concentrations ranging from 0.5 to 5 mg/mL had no significant effect on cell viability, whereas cell viability significantly decreased after treatment with Huaier at concentrations greater than 6 mg/mL ( p <…；Conclusions The present study demonstrated that Huaier alleviates renal tubular epithelial cell apoptosis and protects against DN, partly through inhibition of the Wnt/β‐catenin pathway.
局限
（原文未给出可直接抽取的对应表述）
对本项目的价值
临床前机制证据，需评估物种外推与剂量可行性：代谢—器官轴是干预安全性的主要约束面。 ｜ 价值分 1.7/5（★★☆☆☆）
全文来源
PubMed PMC JATS 全文（正文 34,576 字符，已过 DOI 逐字 + 题名指纹双重归属校验）
原文题录
- 英文标题：Huaier Attenuates Renal Injury in Type 2 Diabetic Nephropathy by Ameliorating Mitochondrial Dysfunction and Inhibiting Tubular Epithelial Cell Apoptosis.
- 期刊与标识：Journal of diabetes · PMID 42957591 · DOI 10.1111/1753-0407.70290
B 级证据分级：B 级（同行评议临床前研究，主要为动物模型）
本文核心结论来自动物模型或体外细胞学，属临床前证据，不能外推至人体。「延长健康寿命」「改善功能」等表述在小鼠中的意义，与人类临床结局之间仍有距离。
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