时间标签：数据日期 2026-10-31 · 生成时间 2026-11-01 05:30（Asia/Shanghai）｜ 检索窗口 PubMed edat 2026/10/28–2026/10/31 ｜ 情报发生日期 2026-10-31
研究问题
Nevertheless, these probes that rely solely on β-Gal activity can suffer from limited specificity. , The primary challenge in developing fluorescent probes for senescent cells detection is not simply detecting β-Gal activity but distinguishing senescence-associated β-Gal from nonsenescent β-Gal activity in complex biological systems.
方法
Senescence-associated β-galactosidase (SA-β-Gal) is one of the most widely used biomarkers for identifying senescent cells. , Conventional SA-β-Gal staining has been extensively applied to cultured cells and tissue sections, but such a method is labor-intensive, time-consuming, end point-based, and poorly compatible with living cells or in vivo real-time monitoring. − Fluorescent sensors responsive to β-Gal activity have been developed for detecting senescent cells in vitro and in vivo.；Moreover, the enzymatic kinetics assay was conducted and the kinetics parameters including K m and V max were calculated to be 203.4 μmol/L and 0.6432 nmol/min/U β-Gal, respectively ().；Prior to live-cell imaging, the cytotoxicity of ECD-Gal was evaluated using a CCK-8 assay.
核心结论
In contrast, fluorophores without the hemicyanine structure showed significant fluorescence enhancement at pH values above 6.0, which is less suitable for monitoring the lysosomal pH transition associated with cellular senescence (, ).；Meanwhile, its fluorescence intensity at 640 nm gradually increased within the pH range of 4.0–6.0 and reached a plateau at pH values above 6.0 (c,d).；Upon incubation with β-Gal under physiological conditions (pH 7.4, 37 °C), the fluorescence intensity of ECD-Gal at 640 nm gradually increased over time (a,b), indicating β-Gal-triggered activation of the sensor. ‖ 结论：These findings demonstrate that combining enzymatic activity with lysosomal microenvironmental information is an effective strategy for improving fidelity in senescence cell sensing.
局限
全文未设独立 limitations 节；结构上为非 RCT 设计，因果推断强度受限。
对本项目的价值
★☆☆☆☆ 细胞衰老清除/重编程是项目干预管线的核心支柱之一。
全文来源
PMC JATS 全文（正文 21,949 字符，已过标题/DOI 逐字归属校验）
原文题录
- 英文标题：A Dual-Locked β‑Galactosidase/pH Near-Infrared Sensor Enables High-Fidelity Monitoring of Therapy-Induced Senescence.
- 期刊与标识：JACS Au · PMID 42953165 · DOI 10.1021/jacsau.6c01028
A 级证据分级：A 级（同行评议人体研究）
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