时间标签：数据日期 2026-11-01 · 生成时间 2026-11-01 05:30（Asia/Shanghai）｜ 检索窗口 PubMed edat 2026/10/31–2026/11/01 ｜ 情报发生日期 2026-11-01
证据级别：摘要级。本条依据 PubMed 结构化摘要与元数据撰写，付费墙全文未获取、未逐节核验，本站未做任何数值推算补全。
研究问题
BACKGROUND During the osteogenic differentiation of human mesenchymal stem cells (hMSCs), amorphous calcium phosphate (ACP) precursors within mitochondria are transported to the extracellular matrix via PINK1/Parkin-mediated mitochondrial…
方法
MATERIAL AND METHODS hMSCs were treated with shRNA to knock down Drp1 or with pharmacological inhibitors (Mdivi-1, CsA).
核心结论
RESULTS Following osteogenic induction, Drp1 Ser616 phosphorylation increased while Ser637 phosphorylation decreased; mitochondrial fission increased, producing fragments approximately 450 nm in diameter, matching the size of autolysosomes, which facilitates…；Inhibition of Drp1 resulted in abnormal mitochondrial elongation, ACP accumulation, and reduced ATP production (decreased to 59.2% in the sh-Drp1 group), and significantly suppressed RUNX2 and ALPL expression as well as mineralization (mineralization area…；In zebrafish, treatment with Mdivi-1 or CsA similarly led to reduced craniofacial mineralization and downregulation of osteogenic gene expression.；CONCLUSIONS Drp1-mediated mitochondrial fission strikes a balance between the efficient transport of ACP precursors and mitochondrial functional homeostasis via the fission-clearance-regeneration cycle.
局限
Further investigation of Drp1-associated mitochondrial dynamics in bone metabolic disease models is warranted.
对本项目的价值
临床前机制证据，需评估物种外推与剂量可行性：线粒体是衰老干预的共同下游效应器，可用于跨器官效应的统一读数。 ｜ 价值分 3.6/5（★★★★☆）
全文来源
未取到全文 → 本卡为摘要级解读，未核验全文
原文题录
- 英文标题：Drp1-Mediated Mitochondrial Fission Coordinates Mineral Precursor Transport and Mitochondrial Homeostasis During Osteogenesis.
- 期刊与标识：Medical science monitor : international medical journal of experimental and clinical research · PMID 42963854 · DOI 10.12659/msm.953980
A 级证据分级：A 级（同行评议人体研究）
本文属人体研究证据，但需注意：观察性或小样本干预的结论不等于已证实临床获益；效应量与长期安全性仍需更大规模、更长随访的试验验证。
关于本文的文献来源：本站依据 IMA 知识库「五刊监控·深度文献卡片」（SOP §4.2 摘要级）撰写，依据期刊公开摘要与 PubMed 元数据；付费墙全文未获取之处已标注「待补全文」，未做任何数值推算补全。