数据日期 2026-10-29 · 生成时间 2026-10-29 06:03
研究问题
They do not, however, establish whether age-associated states are induced by denervation, reversed during regeneration or coordinated with reinnervation.
方法
Models for this mixed-modality cohort included sex and donor nucleus fraction as covariates, with cell-only and nucleus-only sensitivity analyses.；GSE183802 contained 26,031 retained nuclei from six animals and provided a replicated innervated-versus-denervated comparison within author-defined compartments [36].；Six young animals were available only through three multiplexed SRA gene-expression libraries and three paired cell-multiplexing libraries, precluding a balanced local age-by-time analysis.；The conservative primary branch contained 654,053 profiles, but inference used donors, animals, libraries, pools or processed condition aggregates.
核心结论
It contained 24 geriatric libraries or pools spanning days 0, 1, 2, 3.5, 5 and 7 after notexin injury, together with eight young libraries at days 1 and 3.5.；The strongest nominal programme interaction was myogenic denervation sensitivity: estimate standardised-score units, pointwise 95% confidence interval 0.243 to 1.210, , q = 0.226, with four libraries in each of the four age-by-day groups. ‖ 结论：These results establish an auditable comparative resource, not NMJ functional recovery, conserved cross-species signalling or a causal requirement for compartmental coordination.
局限
NMJ-related transcripts were analysed, but their abundance does not establish whether a motor axon occupies an endplate.；In type IIB nuclei, Mtor, Rptor, Akt1 and Tsc2 transcripts increased in the exploratory extraction, but this combination does not establish either complex’s kinase activity: an inhibitor and pathway components can change together without specifying net signalling.；The female-only geriatric injury and male-only denervation experiments cannot identify sex effects, and the exploratory intercostal sex analysis lacks corrected support and does not establish sex-dependent atrophy or inflammation.
对本项目的价值
★★★★☆ 衰弱/肌少症是少有的已有监管认可终点的老年综合征，是临床转化的最短路径。命中核心 线粒体；设计 横断面、单细胞。
全文来源
PMC JATS 全文（正文 66,091 字符，已过标题/DOI 逐字归属校验）
原文题录
- 英文标题：Compartment-Specific Transcriptional Remodelling Defines Denervation and Geriatric Muscle Regeneration.
- 期刊与标识：Int J Mol Sci · PMID 42941799 · DOI 10.3390/ijms27209025**
A 级证据分级：A 级（同行评议人体研究）
本文属人体研究证据，但需注意：观察性或小样本干预的结论不等于已证实临床获益；效应量与长期安全性仍需更大规模、更长随访的试验验证。
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