数据日期 2026-10-29 · 生成时间 2026-10-29 06:03
研究问题
In the present study, we investigated the skin microbiota of a total of 304 healthy volunteers from two distinct age groups using 16S rRNA sequencing.
方法
In the present study, we investigated the skin microbiota of a total of 304 healthy volunteers from two distinct age groups using 16S rRNA sequencing.；OMVs derived from B. fragilis or EVs from S. epidermidis (10 μg in 100 μL volume of PBS) were administered to the skin surface 2 h prior to the application of bacteria (n = 4 for each group).；The strains used in this study were randomly selected.
核心结论
Statistical analysis is by unpaired two-tailed t test. ∗∗∗∗p < 0.0001.；B. fragilis was grown anaerobically in BHI-S (BHI with 0.5% yeast extract and 0.1% cysteine) for 24 h. ‖ 结论：Our comparative microbiota analyses revealed that Bacteroidota, despite their low relative abundance, exert non-negligible functional influence on skin microbial ecology.
局限
Future work will focus on identifying the specific OMV-associated components released by skin-isolated Bacteroides species that initiate autophagosome formation, through integrated proteomic and lipidomic profiling of purified OMVs.；However, 16S rRNA gene sequencing cannot establish the viability, species identity, stable colonization, or anatomical origin of the detected Bacteroides signal; transient environmental deposition and gut-to-skin transfer of Bacteroides species, therefore, cannot be excluded.；Here, we propose a preliminary model in which the anaerobic bacterium Bacteroides induces autophagy through the release of OMVs to clear Pseudomonadota from the skin.
对本项目的价值
★★★☆☆ 皮肤是唯一可直接局部给药、可活检、可影像量化的器官，最快可验证 senotherapeutic 概念。命中核心 微生物组；最大样本量约 154；设计 横断面、多组学。
全文来源
PMC JATS 全文（正文 61,303 字符，已过标题/DOI 逐字归属校验）
原文题录
- 英文标题：Bacteroides fragilis outer membrane vesicle mediates Pseudomonadota colonization by inducing autophagy on the skin.
- 期刊与标识：iScience · PMID 42935965 · DOI 10.1016/j.isci.2026.117969**
B 级证据分级：B 级（同行评议临床前研究，主要为动物模型）
本文核心结论来自动物模型或体外细胞学，属临床前证据，不能外推至人体。「延长健康寿命」「改善功能」等表述在小鼠中的意义，与人类临床结局之间仍有距离。
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