时间标签：数据日期 2026-11-05 · 生成时间 2026-11-05 05:30（Asia/Shanghai）｜ 检索窗口 PubMed edat 2026/11/02 – 2026/11/06 ｜ 情报发生日期 2026-11-05
研究问题
(2019), with modifications to accommodate different experimental objectives specific to the postharvest context.；Depending on the experimental objective, oxidative stress may be applied either before imaging (Figure 1a) or after acquisition of a pre-stress fluorescence image (Figure 1b).；Depending on the experimental objective, oxidative stress can be induced either before or after fluorophore application to assess cumulative oxidative effects or monitor ROS dynamics.
方法
Following stress application, fluorescence images are acquired at predefined time intervals and analyzed using the corresponding background correction strategy.Figure 1Experimental workflow for in vivo fluorescence imaging of ROS-associated oxidative responses in a postharvest context.；This assay was used to evaluate signal activation kinetics, detectability, and temporal stability of the signal in the absence of a biological matrix effect.2.4.2Induction of oxidative stress in lettuce leavesOxidative stress was induced in postharvest lettuce leaves using either a chemical or a physical stressor, following the workflow illustrated in Figure 1b.；For all stress treatments, lettuce leaves treated with H2DCF-DA were first imaged prior to stress application to acquire a baseline fluorescence image, which was used as an internal background reference in subsequent image analysis.Chemical oxidative stress was induced by external application of H2O2.
核心结论
Such limited spatial coverage constrains the characterization of the overall oxidative status of the intact plant tissues and hampers a comprehensive understanding of coordinated oxidative stress responses at the organismal level.To overcome these limitations, highly sensitive in vivo optical imaging platforms have been designed and have demonstrated their ability to monitor ROS non-invasively at the whole-plant scale (Fichman et al., 2019).；Among these imaging modalities, fluorescence imaging has emerged as a particularly powerful approach for the non-destructive monitoring of ROS using sensitive fluorogenic probes.While this approach has demonstrated strong potential for visualizing ROS dynamics in whole plants, its application in postharvest research remains largely limited.；These results indicate that exogenous H2O2 promoted ROS-associated fluorescence beyond the basal oxidative activity observed in untreated tissues.The subsequent decrease in signal intensity in the control and the gradual stabilization of the fluorescence signal in H2O2-treated samples could be interpreted considering the intrinsic capacity of plants to regulate their oxidative status using antioxidant defense systems, including enzymatic (e.g. superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX)) and non-enzymatic (e.g.
局限
However, fluorescence-based methods are subject to probe-specific limitations.；However, these approaches remain limited to relatively small tissue regions, restricting the investigation of oxidative processes at the whole-organ level (Icha et al., 2017).；Such limited spatial coverage constrains the characterization of the overall oxidative status of the intact plant tissues and hampers a comprehensive understanding of coordinated oxidative stress responses at the organismal level.To overcome these limitations, highly sensitive in vivo optical imaging platforms have been designed and have demonstrated their ability to monitor ROS non-invasively at the whole-plant scale (Fichman et al., 2019).
对本项目的价值
中高价值：属「衰老细胞清除 / SASP 轴」方向；细胞衰老 / senolytics / SASP栏目可用作本项目文献库与综述素材。 ｜ 价值分 3.3/5（★★★☆☆）
全文来源
PubMed PMC JATS（正文 36,265 字符，题名指纹 1.0，已过 PubMed PMCID 官方关联 + 题名指纹 + 正文长度归属校验）
原文题录
- 中文题名：实时监测采收生菜叶片 ROS 氧化反应的活体荧光成像方法
- 英文标题：An in vivo fluorescence imaging approach for real-time monitoring of ROS-associated oxidative responses in harvested lettuce leaves.
- 期刊与标识：Frontiers in plant science · PMID 42973529 · DOI 10.3389/fpls.2026.1923387 · PMC PMC13724335