时间标签：数据日期 2026-10-31 · 生成时间 2026-11-01 05:30（Asia/Shanghai）｜ 检索窗口 PubMed edat 2026/10/28–2026/10/31 ｜ 情报发生日期 2026-10-31
研究问题
Using hADSCs as an MSC paradigm, we aimed to characterize the landscape and plasticity of TE expression in human adult stem cells.；By systematically analyzing TE expression dynamics across these settings, we sought to establish an associative and hypothesis-generating framework for defining TE-associated transcriptional states in hADSCs.
方法
Samples were collected at passages 3, 5, 8, and 11 for subsequent RNA sequencing.；Samples were collected at 0 d and 2 d for each treatment for subsequent RNA sequencing.；Cells at passage 9, 10, and 11 were harvested for subsequent RNA sequencing.；Samples were generated under multiple experimental and biological conditions, including culture medium formulation (serum-containing medium, SM, n = 68; serum-free medium, SFM, n = 27), FGF2 supplementation status (FGF2 + , n = 46; FGF2-, n = 49), donor identity (four independent donors: D1, n = 40; D2, n = 20; D3, n = 24; D4, n = 11), passage-dependent replicative aging ( ≤ P8, n = 48; >P8, n = 47), and senescence-inducing DNA damage (doxorubicin and/or irradiation, n = 12) as shown in Supplementary Table .
核心结论
We performed four experiments (Supplementary Fig. ) and generated four datasets: Medium Experiment 1 (Med1, n = 20), Medium Experiment 2 (Med2, n = 16), DNA Damage Experiment (DD, n = 24), and FGF2 Experiment (FGF, n = 35).；Robust expression of CD105/ENG, CD73/NT5E, and CD90/THY1 together with low expression of CD34, CD14, CD19, CD45/PTPRC, and HLA, supported hADSC identity in all samples (Fig. , Supplementary Fig. ).；SINE and LINE elements were generally more highly expressed, whereas LTR elements showed lower expression levels (Fig. ), consistent with known TE expression characteristics. ‖ 结论：Given the non-orthogonal design, limited donor number, and uneven donor representation across conditions, these results should be viewed as cohort-specific and require confirmation in larger cohorts.
局限
Together, these findings potentially raise an underexplored regulatory dimension in ASCs that warrants further investigation.；Donor heterogeneity remains a central challenge but rarely explored for MSC translation.；We observed donor-associated variability in ASC TE expression patterns, which may be relevant to future efficacy- and safety-oriented evaluations pending functional validation and warrants further investigation in larger cohorts.
对本项目的价值
★☆☆☆☆ 干细胞衰竭与部分重编程决定 rejuvenation 路线的可行边界与安全上限。
全文来源
PMC JATS 全文（正文 40,799 字符，已过标题/DOI 逐字归属校验）
原文题录
- 英文标题：The landscape and plasticity of transposable element expression in an adult stem cell of high physiological and clinical significance.
- 期刊与标识：Cell Death Discov · PMID 42957621 · DOI 10.1038/s41420-026-03277-7
A 级证据分级：A 级（同行评议人体研究）
本文属人体研究证据，但需注意：观察性或小样本干预的结论不等于已证实临床获益；效应量与长期安全性仍需更大规模、更长随访的试验验证。
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