数据日期 2026-10-29 · 生成时间 2026-10-29 06:03
研究问题
Skin aging is a complex process caused by the cumulative effects of intrinsic and extrinsic stress.
方法
Cell Viability Assay] Cell viability was measured using the EZ-Cytox assay kit (DoGenBio, Seoul, Republic of Korea).；HDFn were seeded in 96-well plates at a 0.8 × 104 per well and treated with samples for 24 h.；After incubation for 1 h at 37 °C in a 5% CO2 incubator, the absorbance was measured at 450 nm using a microplate reader (Tecan, Mannedorf, Switzerland).；Experimental Design and Treatment G] Cells were divided into four groups: (1) normal control (no UVB, no treatment), (2) UVB control (UVB only), (3) UVB + Paeonol 50 µM, and (4) UVB + Paeonol 100 µM.
核心结论
UVA accounts for approximately 95% of the solar UV that reaches the Earth’s surface and penetrates deeply into the reticular dermis.；In addition, paeonol alone has been reported to attenuate H2O2-induced senescence in human fetal lung fibroblasts, reducing SA-β-gal positive cells and intracellular ROS.；Paeonol Suppresses UVB-Induced Senescence Markers and Senescent Cell Burden To evaluate the effect of paeonol on cellular senescence at the phenotypic level, SA-β-gal staining was performed using 100 µM paeonol, the most effective concentration in the preceding experiments. ‖ 结论：We assessed well-established indicators of cellular senescence.
局限
Although we also observed reductions in SASP factors following paeonol treatment, the extent to which these effects are mediated by JNK/AP-1 signaling remains to be established.；The translational relevance of these findings remains to be established because the present study employed an in vitro UVB-SIPS model that does not fully recapitulate physiological responses to solar UV radiation in vivo.
对本项目的价值
★★★☆☆ 细胞衰老清除/重编程是项目干预管线的核心支柱之一。命中核心 细胞衰老；最大样本量约 1。
全文来源
PMC JATS 全文（正文 25,978 字符，已过标题/DOI 逐字归属校验）
原文题录
- 英文标题：Paeonol Attenuates UVB-Induced Premature Senescence in Human Dermal Fibroblasts to Promote Skin Longevity.
- 期刊与标识：Int J Mol Sci · PMID 42942117 · DOI 10.3390/ijms27209352**
A 级证据分级：A 级（同行评议人体研究）
本文属人体研究证据，但需注意：观察性或小样本干预的结论不等于已证实临床获益；效应量与长期安全性仍需更大规模、更长随访的试验验证。
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