时间标签：数据日期 2026-10-31 · 生成时间 2026-11-01 05:30（Asia/Shanghai）｜ 检索窗口 PubMed edat 2026/10/28–2026/10/31 ｜ 情报发生日期 2026-10-31
研究问题
However, as a systemic drug, metformin has relatively low concentrations in joint tissues, and its pharmacokinetics make it difficult to sustain joint protection (Abbasi et al. ).；However, F127 hydrogel exhibits limited drug release duration due to rapid dissociation in aqueous medium (Li and Mooney, ).
方法
By performing extraction with saturated NaCl and concentrating with cold anhydrous ethanol, we obtained purified F127-NPC.；For dopamine conjugation, dopamine hydrochloride (1.00 mmol) was first dissolved in 4 mL N, N-dimethylformamide under an N₂ atmosphere, followed by the addition of TEA (324 μL) after 20 min of stirring.；The cytocompatibility evaluation of F127, F127-DA-HRP hydrogel, and metformin was performed using the Cell Counting Kit-8 (CCK-8) assay.；After 24, 48, or 72 h of incubation, the cells were washed twice with PBS and incubated with CCK-8 reagent (10 µL/well), followed by absorbance measurement using a microplate reader.
核心结论
As expected, the gelation time (31.3 s) and gelation temperature (22.7 °C) of the cold F127-DA-HRP solution were significantly lower than those of the cold F127 solution (53.6 s and 32.8 °C, respectively) (Table S1).；In previous clinical reports, thicker syringe needles, such as 18 G (840 µm inner diameter) or 22 G (410 µm inner diameter), have been utilized to deliver hydrogel systems to cells, which may increase the patient's pain response (Xu et al. ).；The enzyme-catalyzed crosslinking endowed the F127-DA-HRP hydrogel with significantly enhanced mechanical strength compared to the F127 hydrogel (). ‖ 结论：These limitations highlight important directions for further optimization and preclinical evaluation before clinical translation.
局限
In this study, several limitations must be acknowledged.；Such measurements should be incorporated in future studies to strengthen the mechanistic understanding of this system.；Although the hydrogel demonstrated sustained metformin release for up to 30 days in vitro, the lack of robust adhesion to articular cartilage or synovial tissues could compromise its in situ residence time under mechanical loading and synovial fluid turnover.
对本项目的价值
★☆☆☆☆ 炎症衰老是几乎所有慢性老年病的公共通路，也是最有希望做「抗炎但保留免疫」解耦的靶点。
全文来源
PMC JATS 全文（正文 48,255 字符，已过标题/DOI 逐字归属校验）
原文题录
- 英文标题：Sustained release of metformin through an injectable hydrogel for synergistic macrophage reprogramming and ROS scavenging in the analgesic treatment of osteoarthritis.
- 期刊与标识：Drug Deliv · PMID 42952350 · DOI 10.1080/10717544.2026.2738344
B 级证据分级：B 级（同行评议临床前研究，主要为动物模型）
本文核心结论来自动物模型或体外细胞学，属临床前证据，不能外推至人体。「延长健康寿命」「改善功能」等表述在小鼠中的意义，与人类临床结局之间仍有距离。
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