数据日期 2026-10-29 · 生成时间 2026-10-29 06:03
研究问题
Excessive intake of high-fat diets (HFDs) serves as a major driving factor for the growing prevalence of obesity, metabolic disorders, cardiovascular diseases, and various malignant tumors.
方法
This study utilized 40 male C57BL/6 mice, aged 7 weeks, which were purchased from the Animal Research Center of China Three Gorges University and housed at the Animal Center of Yangtze University (Jingzhou, China).；All mice were randomly assigned to either a normal diet group (DC group, n = 8) or a high-fat diet group (HFD group, n = 32) using a simple random sampling method.；After 12 weeks, the mice in the HFD group were further randomly subdivided into four groups (n = 8 per group): a model group (MG group), an exercise group (EG group), an Nrf2 inhibitor group (NG group), and an exercise plus Nrf2 inhibitor group (ENG group).；During the animal treatment, feeding, and sample collection procedures, the researchers were blinded to the group information.
核心结论
During weeks 1–12, the body weight of the HFD groups was significantly higher than that of the DC group (p < 0.05 or p < 0.01), indicating a marked increase in body weight in mice fed with HFD.；In contrast, the MG group displayed loosely arranged muscle cells with numerous intracellular vacuoles and irregular tissue morphology, accompanied by a significant reduction in the proportional area of muscle tissue (p < 0.01). ‖ 结论：In summary, exercise is associated with skeletal muscle autophagy–lysosome-related markers and Nrf2/ARF1 signaling in HFD mice.
局限
Fifth, although the expression and colocalization of key autophagic proteins were examined, direct autophagic flux and lysosomal reformation assays were not conducted, so the conclusions about autophagic activity remain preliminary.
对本项目的价值
★★★★☆ 衰弱/肌少症是少有的已有监管认可终点的老年综合征，是临床转化的最短路径。命中核心 蛋白稳态/自噬；最大样本量约 32。
全文来源
PMC JATS 全文（正文 35,408 字符，已过标题/DOI 逐字归属校验）
原文题录
- 英文标题：Exercise Is Associated with Skeletal Muscle Autophagy-Lysosome-Related Markers and Nrf2/ARF1 Signaling in HFD Mice.
- 期刊与标识：Nutrients · PMID 42942899 · DOI 10.3390/nu18203329**
B 级证据分级：B 级（同行评议临床前研究，主要为动物模型）
本文核心结论来自动物模型或体外细胞学，属临床前证据，不能外推至人体。「延长健康寿命」「改善功能」等表述在小鼠中的意义，与人类临床结局之间仍有距离。
关于本文的文献来源：本站依据 IMA 知识库「五刊监控·深度文献卡片」撰写，依据已获取的期刊全文（方法、样本量与结论已回到原文核对）；未能获取全文的同批条目另行标注「摘要级」，全程未做任何数值推算补全。