时间标签：数据日期 2026-10-31 · 生成时间 2026-11-01 05:30（Asia/Shanghai）｜ 检索窗口 PubMed edat 2026/10/28–2026/10/31 ｜ 情报发生日期 2026-10-31
研究问题
Fracture can be inflicted by minor force and evolve to bone defects as the shifting osteogenesis-adipogenesis promotes adiposity , which cannot effectively heal the fracture gap and remodel it into mature bone.；However, the effects of electrical stimulation on the tilted bone-fat balance in an inflammatory environment have not been studied.；However, PCL is not electrically conductive, and only the glycine grains exposed on the surface are effective.
方法
This was followed by PEG concentration to obtain a 10% RSF solution.；The viability was assessed on days 1, 3, and 5 by the Live/Dead Assay (Yeasen, 40747ES76).；RT-qPCR was performed by mixing cDNA, primers, and SYBR Green Master Mix (Yeasen, 11202ES08).；After continuous piezoelectric stimulation by MXene/PVDF(3)/RSF hydrogel for 7 and 14 days, cells were fixed with 4% paraformaldehyde or 75% alcohol and stained with ALP staining solution (Beyotime, C3206) or ARS staining solution (Beyotime, C0148S) for 1 h.
核心结论
The FT-IR spectra of the PVDF nanofiber membrane are shown (B), where there are more pronounced absorption peaks at 841, 1275, and 1434 cm−1, indicating a higher proportion of the β-crystalline phases in the structure after electrospinning .；During electrospinning, PVDF is stretched to form more β-crystalline phases compared to electrospraying, and the piezoelectric performance can be adjusted by simply inserting more or fewer layers .；Adding MXene resulted in a uniform, smaller pore size and a denser hydrogel structure, suggesting that the crystallization of ice was limited as MXene nanosheets bound the RSF fibrils . ‖ 结论：These results indicate that piezoelectricity can effectively rebalance bone-fat balance by enhancing osteoblast differentiation, reducing adipogenesis, and suppressing osteoclastogenesis at the same time.
局限
The limitation is that Pla2g4a was initially explored using the pre-osteoblast MC3T3 cell line; the gene and metabolite profile could not directly recapitulate the molecular processes associated with osteogenic–adipogenic lineage commitment of BMSCs.
对本项目的价值
★☆☆☆☆ 衰弱/肌少症是少有的已有监管认可终点的老年综合征，是临床转化的最短路径。
全文来源
PMC JATS 全文（正文 48,986 字符，已过标题/DOI 逐字归属校验）
原文题录
- 英文标题：Sandwich-structured PVDF/protein piezoelectric hydrogel regulates osteogenesis and adipogenesis through glycerophospholipid metabolism in osteoporotic inflammatory bone defects.
- 期刊与标识：Mater Today Bio · PMID 42953339 · DOI 10.1016/j.mtbio.2026.103770
B 级证据分级：B 级（同行评议临床前研究，主要为动物模型）
本文核心结论来自动物模型或体外细胞学，属临床前证据，不能外推至人体。「延长健康寿命」「改善功能」等表述在小鼠中的意义，与人类临床结局之间仍有距离。
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