时间标签：数据日期 2026-11-03 · 生成时间 2026-11-03 05:30（Asia/Shanghai）｜ 检索窗口 PubMed edat 2026/10/31 – 2026/11/03 ｜ 情报发生日期 2026-11-03
研究问题
We hypothesized that calcium signaling regulated by vitamin D may be involved in sarcopenia and could influence the effectiveness of vitamin D treatment.；We further investigated whether these changes contribute to the reduced therapeutic efficacy of vitamin D in treating sarcopenia, using TNF-a- treated C2C12 myoblasts as an in vitro model.；To investigate the activation of the calcium signaling pathway, transcriptomic and proteomic datasets (GSE225576 and PXD040722) from mice aged 6, 15, 24, and 30 mo were obtained (18).
方法
Calcium signaling pathway activity was analyzed through bioinformatics, and its regulation was validated in an in vitro model of sarcopenia using TNF-a-treated C2C12 cells.；In this study, we analyzed calcium signaling pathway activity using transcriptomic and proteomic datasets from aged mouse muscle (18), and identified a decrease in CamkIIa and an increase in CamkIIb expression in aging tissues.；For transfection, C2C12 cells were seeded at 33104 cells per well in 12-well plates.
核心结论
Collectively, these findings suggest that the downregulation of Camk2a and upregulation of Camk2b may contribute to vitamin D treatment resistance in sarcopenia-related muscle atrophy, potentially via NF-kB activation and SASP expression driven by Camk2b.；These findings suggest that vitamin D-regulated cal- cium homeostasis may play a pivotal role in sarcopenia pathogenesis.；These findings suggest that although the overall enrichment of the calcium signal- ing pathway did not significantly change with age, the pathway may be extensively dysregulated due to differ- ential expression of key regulators such as Camk2a and Camk2b.
局限
However, effective treat- ment options remain limited, with resistance training and adequate nutrient intake currently regarded as the most effective interventions.；A recent meta-analysis con- cluded that vitamin D had minimal impact on appen- *To whom correspondence should be addressed.
对本项目的价值
中等价值：属「衰老细胞清除 / SASP 轴」方向；肌肉骨骼 / 衰弱 / 肌少症栏目可用作本项目文献库与综述素材。 ｜ 价值分 2.6/5（★★★☆☆）
全文来源
出版商/仓储 OA PDF（正文 41,536 字符，题名指纹 1.00，已过 DOI 逐字 + 题名指纹 + 正文长度三重归属校验）
原文题录
- 中文题名：Camk2a 不足与 Camk2b 过剩：肌少症体外模型维生素 D 无应答的关键
- 英文标题：Inadequate Camk2a and Excess Camk2b: The Key to Vitamin D Irresponsiveness in an In Vitro Model of Sarcopenia.
- 期刊与标识：J Nutr Sci Vitaminol (Tokyo) · PMID 42968240 · DOI 10.3177/jnsv.72.361 · EDAT 2026-11-01