时间标签：数据日期 2026-10-31 · 生成时间 2026-11-01 05:30（Asia/Shanghai）｜ 检索窗口 PubMed edat 2026/10/28–2026/10/31 ｜ 情报发生日期 2026-10-31
研究问题
However, whether the cochlear immune microenvironment differs between these conditions, and whether it can be thera- peutically modulated, remains largely unknown.；However, accumulating evidence indicates that the cochlea actively engages in immune and inflammatory processes, suggesting that immune regulation plays a critical role in both cochlear physiology and pathology [2].；However, the specific types of immune cells involved in the patho- logical mechanisms of NIHL and ARHL, as well as their interactions, remain largely unknown.
方法
Iba1⁺ microglia-like cells and Sox2⁺ SGCs were systematically characterized across four groups: young control (CTRL), young NIHL, aged vehicle-treated (Veh), and aged PLX5622-treated (PLX) mice, using double immunofluorescence staining for Iba1, Tmem119, Sox2, and CD74.；In contrast, aged Veh mice showed a significant increase in total Iba1⁺ cell density and in Iba1+ /Tmem119+ , Iba1⁺/ CD74⁺ and Sox2⁺/CD74⁺ cell densities relative to CTRL, with a similar upward trend for Sox2⁺/Tmem119⁺ cells that did not reach statistical significance after multiple- comparison correction.；Double immunofluorescence (IF) staining for Iba1, Tmem119, Sox2, and CD74 revealed age-associated differences in marker co- expression patterns within the SG and changes in selected marker-positive cell populations following PLX5622 treatment in aged mice.
核心结论
In young mice, NIHL did not significantly alter total Iba1⁺ cell density or Iba1⁺/ CD74⁺ co-expression in the SG compared to CTRL.；In contrast, aged Veh mice showed a significant increase in total Iba1⁺ cell density and in Iba1+ /Tmem119+ , Iba1⁺/ CD74⁺ and Sox2⁺/CD74⁺ cell densities relative to CTRL, with a similar upward trend for Sox2⁺/Tmem119⁺ cells that did not reach statistical significance after multiple- comparison correction.；A 7-day course of the PLX5622 significantly reduced total Iba1⁺, Iba1+ /Tmem119+ , Iba1⁺/CD74⁺, and Sox2⁺/CD74⁺ cell densities in aged PLX mice. ‖ 结论：We therefore restrict our conclusions to the immu- noreactivity level and we do not claim that these changes constitute functional remodeling of MLCs or SGCs identity or activity.
局限
Although age and genotype were confounded and PLX5622 may exert both local and systemic effects, these observations highlight the coch- lear SG immune microenvironment as a potential area for further investigation in ARHL.；We acknowledge this lack of randomization as a methodological limitation.；This potential confound for neuroimmune endpoints is considered further in the Discussion.
对本项目的价值
★☆☆☆☆ 脑老化是最大未满足需求，也是终点最难量化的领域；biomarker 与认知读数选择最敏感。
全文来源
出版商 OA PDF（正文 75,793 字符，已过 DOI 逐字 / 标题指纹归属校验）
原文题录
- 英文标题：Age-associated changes in microglia-like and satellite glial cell markers in the cochlear spiral ganglion.
- 期刊与标识：Geroscience · PMID 42955659 · DOI 10.1007/s11357-026-02617-w
B 级证据分级：B 级（同行评议临床前研究，主要为动物模型）
本文核心结论来自动物模型或体外细胞学，属临床前证据，不能外推至人体。「延长健康寿命」「改善功能」等表述在小鼠中的意义，与人类临床结局之间仍有距离。
关于本文的文献来源：本站依据 IMA 知识库「五刊监控·深度文献卡片」撰写，依据已获取的期刊全文（方法、样本量与结论已回到原文核对）；未能获取全文的同批条目另行标注「摘要级」，全程未做任何数值推算补全。